EFFECT OF FUSION DURATION ON CLEAVAGE AND DEVELOPMENTAL RATE OF ELECTROFUSED TETRAPLOID 2-CELL BOVINE EMBRYO

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DARABI M.R.; NASRESFAHANI MOHAMMAD HOSSEIN

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Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2005 | Volume:8 | Issue:2 (31) Page(s): 15-23

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Title

EFFECT OF FUSION DURATION ON CLEAVAGE AND DEVELOPMENTAL RATE OF ELECTROFUSED TETRAPLOID 2-CELL BOVINE EMBRYO

Author(s)

DARABI M.R. | NASRESFAHANI MOHAMMAD HOSSEIN | Issue Writer Certificate

Keywords

FUSION TIMEQ4

BLASTOCYSTQ3

TETRAPLOIDQ4

BOVINEQ4

Abstract

Introduction: Because TETRAPLOID embryo is used as a base for growth and development of transgenic cells, one of the most important stages in animal biotechnology is to produce TETRAPLOIDy by electro fused 2-cell embryo. The aim of this study was to determine the effect of fusion duration on developmental rate of TETRAPLOID embryos.Materials and Methods: In this experimental study some of the BOVINE 2-cell embryos were obtained from in vitro matured and fertilized cumulus oocyte complexes 33-35 hr post fertilization as an unexposed control group (UCG). The remaining 2-cell embryos were exposed to 0.75 kilovolt per centimeter for 80 microsecond, and were transferred to SOF1 medium. Subsequently those embryos fused at 30 and 60 minute post electrofusion were categorized as fused groups (FG30 and FG60) and separated from unfused embryos as exposed control group (ECG). The developmental rate was compared between UCG, ECG, FG30, and FG60 groups and the relation between fusion duration and cleavage and developmental rate was surveyed.Results: The cleavage rate up to 8-cell stage in FG60 was increased significantly compared to FG30 (p<0.05) while the BLASTOCYST rate has no significant difference between the two groups. The cleavage and developmental rate in UCG was significantly higher than ECG, FG60 and FG30. Chromosomal analysis showed that 76% of embryos were true TETRAPLOID.Conclusion: The fused embryos in FG60had more ability to produce embryos up to 8-cell stage than FG30. The electrical pulse can decrease the cleavage and developmental ability of embryo.

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تاثیر تغییرات ولتاژ و زمان فیوژن بر تسهیم جنینهای تتراپلوییدی حاصل از الکتروفیوژن جنینهای دو سلولی گاو

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APA: Copy

DARABI, M.R., & NASRESFAHANI, MOHAMMAD HOSSEIN. (2005). EFFECT OF FUSION DURATION ON CLEAVAGE AND DEVELOPMENTAL RATE OF ELECTROFUSED TETRAPLOID 2-CELL BOVINE EMBRYO. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 8(2 (31)), 15-23. SID. https://sid.ir/paper/69740/en

Vancouver: Copy

DARABI M.R., NASRESFAHANI MOHAMMAD HOSSEIN. EFFECT OF FUSION DURATION ON CLEAVAGE AND DEVELOPMENTAL RATE OF ELECTROFUSED TETRAPLOID 2-CELL BOVINE EMBRYO. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2005;8(2 (31)):15-23. Available from: https://sid.ir/paper/69740/en

IEEE: Copy

M.R. DARABI, and MOHAMMAD HOSSEIN NASRESFAHANI, “EFFECT OF FUSION DURATION ON CLEAVAGE AND DEVELOPMENTAL RATE OF ELECTROFUSED TETRAPLOID 2-CELL BOVINE EMBRYO,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 8, no. 2 (31), pp. 15–23, 2005, [Online]. Available: https://sid.ir/paper/69740/en

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