DETERMINATION OF ISOLATED BRUCELLA SPECIES IN BLOOD SAMPLES OF PATIENTS WITH BRUCELLOSIS BY PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NASERI Z.; YOUSEF ALIKHANI M.; HASHEMI H.; ARABESTANI M.R.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2014 | Volume:17 | Issue:5 (86) Page(s): 52-59

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,748

Download:

Cites:

Information Journal Paper

Title

DETERMINATION OF ISOLATED BRUCELLA SPECIES IN BLOOD SAMPLES OF PATIENTS WITH BRUCELLOSIS BY PCR

Author(s)

NASERI Z. | YOUSEF ALIKHANI M. | HASHEMI H. | ARABESTANI M.R. | Issue Writer Certificate

Keywords

BRUCELLOSISQ2

BLOOD CULTUREQ2

DIAGNOSISQ3

PCRQ2

Abstract

Background: BRUCELLOSIS is a major cause of zoonosis disease and is endemic in hamadan Province in Iran. The purpose of this study was to isolate Brucella species from BRUCELLOSIS patients and identify different species of this bacterium in order to determine the prevalence of the species.Materials and Methods: This study was descriptive- cross sectional and fifty blood samples were obtained from BRUCELLOSIS patients with clinical symptoms. The samples were cultured in BACTEC system and incubated for 14 days. Then, the samples were cultured on Brucella agar and biochemical tests were done for identification of bacteria. Finally, Polymerase Chain Reaction (PCR) applied for confirmation and isolated identification with specific primers.Results: Seven Brucella strains were isolated from 50 blood samples of the patients with BRUCELLOSIS by BLOOD CULTURE and PCR. The PCR results on blood specimens showed 4 positive in spite of the negative results of BLOOD CULTURE. PCR and biochemical methods revealed that all the 11 isolated bacteria were Brucella Melitensis.Conclusion: This study was designed to evaluate PCR technique as a diagnostic tool for brucellaspp in comparison to conventional techniques. This study showed a high prevalence of BRUCELLOSIS due to Brucella Melitensis in Hamadan Province and efforts in this region should be aimed at the eradication of this bacterium.

Cites

No record.

References

Cite

APA: Copy

NASERI, Z., YOUSEF ALIKHANI, M., HASHEMI, H., & ARABESTANI, M.R.. (2014). DETERMINATION OF ISOLATED BRUCELLA SPECIES IN BLOOD SAMPLES OF PATIENTS WITH BRUCELLOSIS BY PCR. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 17(5 (86)), 52-59. SID. https://sid.ir/paper/69848/en

Vancouver: Copy

NASERI Z., YOUSEF ALIKHANI M., HASHEMI H., ARABESTANI M.R.. DETERMINATION OF ISOLATED BRUCELLA SPECIES IN BLOOD SAMPLES OF PATIENTS WITH BRUCELLOSIS BY PCR. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2014;17(5 (86)):52-59. Available from: https://sid.ir/paper/69848/en

IEEE: Copy

Z. NASERI, M. YOUSEF ALIKHANI, H. HASHEMI, and M.R. ARABESTANI, “DETERMINATION OF ISOLATED BRUCELLA SPECIES IN BLOOD SAMPLES OF PATIENTS WITH BRUCELLOSIS BY PCR,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 17, no. 5 (86), pp. 52–59, 2014, [Online]. Available: https://sid.ir/paper/69848/en

Related Journal Papers