Inhibitor of Multi-cyclin-dependent Kinases (AT7519) Reduced Survival of U937 Leukemic Cells and Enhanced Anti-leukemic Effect of Vincristine: A Highlight to CDK Inhibition Efficacy in Acute Leukemia

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Moradi Moraddahande Fateme; Shameli Houjghan Mona; Yousefi Amir Mohammad; Safaroghli Azar Ava; POURBAGHERI SIGAROODI ATIEH; BASHASH DAVOOD

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Journal: International Journal of Cancer Management Year:2021 | Volume:14 | Issue:2 Page(s): 0-0

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Title

Inhibitor of Multi-cyclin-dependent Kinases (AT7519) Reduced Survival of U937 Leukemic Cells and Enhanced Anti-leukemic Effect of Vincristine: A Highlight to CDK Inhibition Efficacy in Acute Leukemia

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Keywords

Acute Myeloid Leukemia

Cyclin-dependent Kinases

U937 Cells

Cell Cycle

Vincristine

Abstract

Background: The conservative character of the Cell Cycle outlined that any dysregulation in the regulatory components of this process in normal cells opens a gate toward neoplastic transformation. Objectives: Given the critical role of Cyclin-dependent Kinases (CDKs) in cancer pathogenesis and based on their frequent aberrancy in human leukemia, the present study aimed at evaluating the suppressive effect of a multi-CDK inhibitor AT7519 on Acute Myeloid Leukemia-derived U937 Cells. Methods: To assess the anti-leukemic effects of the inhibitor on Acute Myeloid Leukemia (AML) cells, we used MTT and trypan blue assays. Flow cytometric analysis and q-RT-PCR were also applied to evaluate the impact of AT7519 on Cell Cycle and apoptosis. Results: The results suggested that suppression of CDK in U937 Cells hampered the proliferation of leukemic cells through a G2/M arrest mediated by p21 gene. Additionally, the anti-survival impact of AT7519 on these cells was shown to be along with the apoptosis initiation not only through the increment of pro-apoptotic gene expression but also through diminishing the mRNA levels of both Pin1 and Survivin. Notably, the potent anti-leukemic property of this agent has become more prominent when we found that the blockage of CDKs in AML cells could synergize with the cytotoxic effect of Vincristine (VCR). To the best of our knowledge, little is known about the molecular mechanisms of resistance to AT7519 and we proposed that the effectiveness of this agent was partially attenuated through either c-Myc or autophagy activation in U937 Cells. Conclusions: This study suggests that the pharmacological targeting of CDKscould probablyunwindthe complexity of therapeutic obstacles on the way of acute leukemia, either in the context of mono-or combined-modal strategy.

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APA: Copy

Moradi Moraddahande, Fateme, Shameli Houjghan, Mona, Yousefi, Amir Mohammad, Safaroghli Azar, Ava, POURBAGHERI SIGAROODI, ATIEH, & BASHASH, DAVOOD. (2021). Inhibitor of Multi-cyclin-dependent Kinases (AT7519) Reduced Survival of U937 Leukemic Cells and Enhanced Anti-leukemic Effect of Vincristine: A Highlight to CDK Inhibition Efficacy in Acute Leukemia. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 14(2), 0-0. SID. https://sid.ir/paper/699840/en

Vancouver: Copy

Moradi Moraddahande Fateme, Shameli Houjghan Mona, Yousefi Amir Mohammad, Safaroghli Azar Ava, POURBAGHERI SIGAROODI ATIEH, BASHASH DAVOOD. Inhibitor of Multi-cyclin-dependent Kinases (AT7519) Reduced Survival of U937 Leukemic Cells and Enhanced Anti-leukemic Effect of Vincristine: A Highlight to CDK Inhibition Efficacy in Acute Leukemia. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2021;14(2):0-0. Available from: https://sid.ir/paper/699840/en

IEEE: Copy

Fateme Moradi Moraddahande, Mona Shameli Houjghan, Amir Mohammad Yousefi, Ava Safaroghli Azar, ATIEH POURBAGHERI SIGAROODI, and DAVOOD BASHASH, “Inhibitor of Multi-cyclin-dependent Kinases (AT7519) Reduced Survival of U937 Leukemic Cells and Enhanced Anti-leukemic Effect of Vincristine: A Highlight to CDK Inhibition Efficacy in Acute Leukemia,” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 14, no. 2, pp. 0–0, 2021, [Online]. Available: https://sid.ir/paper/699840/en

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