MELATONIN PRETREATED BLASTOCYSTS ALONG WITH CALCITONIN ADMINISTRATION IMPROVED IMPLANTATION BY UPREGULATION OF HEPARIN BINDING-EPIDERMAL GROWTH FACTOR EXPRESSION IN MURINE ENDOMETRIUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOGHANI GHOROGHI FATEMEH; MOSHKDANIAN GHAZALE; SEHAT MOJTABA; NEMATOLLAHI MAHANI SEYED NOUREDDIN; RAGERDI KASHANI IRAJ; PASBAKHSH PARICHEHR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2018 | Volume:19 | Issue:4 Page(s): 599-606

Download Full-Text

View:

287

Download:

150

Cites:

Information Journal Paper

Title

MELATONIN PRETREATED BLASTOCYSTS ALONG WITH CALCITONIN ADMINISTRATION IMPROVED IMPLANTATION BY UPREGULATION OF HEPARIN BINDING-EPIDERMAL GROWTH FACTOR EXPRESSION IN MURINE ENDOMETRIUM

Author(s)

Keywords

BLASTOCYST

CALCITONIN

HB-EGF

IMPLANTATION

MELATONIN

Abstract

Objective: IMPLANTATION failure is an obstacle in assisted reproduction techniques (ART). CALCITONIN is a molecules involved in uterine receptivity and embryo IMPLANTATION. MELATONIN can promote embryo quality and improve IMPLANTATION. This study examines the effect of pretreatment of BLASTOCYSTs with MELATONIN and CALCITONIN on heparin binding-epidermal growth factor (HB-EGF) expression in murine endometrium.Materials and Methods: In this experimental study, we collected 2-cell embryos from the oviducts of 1.5 day pregnant NMRI mice. Embryos were cultured to the BLASTOCYST in GTM medium with or without 10-9 M MELATONIN. Pregnant and pseudo-pregnant mice received intraperitoneal (IP) injections of 2 IU CALCITONIN. After 24 hours, we transferred the cultured BLASTOCYSTs into the uteri of pseudo-pregnant mice. Two days later, IMPLANTATION sites were counted and we assessed the levels of HB-EGF mRNA and protein in the uteri of naturally pregnant and pseudo-pregnant mice by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Statistical analysis was performed with one-way ANOVA followed by the Tukey post hoc test. P<0.05 was considered statistically significant.Results: MELATONIN pretreatment of BLASTOCYSTs along with CALCITONIN administration significantly increased HB-EGF mRNA and protein (P<0.001) in the endometrium of pseudo-pregnant mice. Administration of CALCITONIN in naturally pregnant mice significantly increased HB-EGF mRNA and protein levels (P<0.001). Compared with the control group (2.6 ± 0.5), the average number of IMPLANTATION sites in the MELATONIN group (4.6 ± 0.5, P<0.05) and CALCITONIN group (7 ± 1, P<0.001) significantly increased. There was a significant increase in IMPLANTATION sites in the combined MELATONIN and CALCITONIN group (8.6 ± 0.5, P<0.001). CALCITONIN significantly enhanced CALCITONIN receptor mRNA (P<0.001) and protein (P<0.05) in the uteri of naturally pregnant and pseudo-pregnant mice.Conclusion: MELATONIN pretreated BLASTOCYSTs along with CALCITONIN increased HB-EGF expression in the uteri of pseudopregnant mice. CALCITONIN administration upregulated HB-EGF in uteri of naturally pregnant mice.

Cites

No record.

References

No record.

Cite

APA: Copy

MOGHANI GHOROGHI, FATEMEH, MOSHKDANIAN, GHAZALE, SEHAT, MOJTABA, NEMATOLLAHI MAHANI, SEYED NOUREDDIN, RAGERDI KASHANI, IRAJ, & PASBAKHSH, PARICHEHR. (2018). MELATONIN PRETREATED BLASTOCYSTS ALONG WITH CALCITONIN ADMINISTRATION IMPROVED IMPLANTATION BY UPREGULATION OF HEPARIN BINDING-EPIDERMAL GROWTH FACTOR EXPRESSION IN MURINE ENDOMETRIUM. CELL JOURNAL (YAKHTEH), 19(4), 599-606. SID. https://sid.ir/paper/701555/en

Vancouver: Copy

MOGHANI GHOROGHI FATEMEH, MOSHKDANIAN GHAZALE, SEHAT MOJTABA, NEMATOLLAHI MAHANI SEYED NOUREDDIN, RAGERDI KASHANI IRAJ, PASBAKHSH PARICHEHR. MELATONIN PRETREATED BLASTOCYSTS ALONG WITH CALCITONIN ADMINISTRATION IMPROVED IMPLANTATION BY UPREGULATION OF HEPARIN BINDING-EPIDERMAL GROWTH FACTOR EXPRESSION IN MURINE ENDOMETRIUM. CELL JOURNAL (YAKHTEH)[Internet]. 2018;19(4):599-606. Available from: https://sid.ir/paper/701555/en

IEEE: Copy

FATEMEH MOGHANI GHOROGHI, GHAZALE MOSHKDANIAN, MOJTABA SEHAT, SEYED NOUREDDIN NEMATOLLAHI MAHANI, IRAJ RAGERDI KASHANI, and PARICHEHR PASBAKHSH, “MELATONIN PRETREATED BLASTOCYSTS ALONG WITH CALCITONIN ADMINISTRATION IMPROVED IMPLANTATION BY UPREGULATION OF HEPARIN BINDING-EPIDERMAL GROWTH FACTOR EXPRESSION IN MURINE ENDOMETRIUM,” CELL JOURNAL (YAKHTEH), vol. 19, no. 4, pp. 599–606, 2018, [Online]. Available: https://sid.ir/paper/701555/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops