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Information Journal Paper

Title

CALCIUM DETERMINATION IN EDTA TREATED PLASMA BY COLORIMETRIC METHOD AND MICROPLATE READING FORMAT

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Keywords

Abstract

 Background: CALCIUM has different functions in metabolic processes and increase ordecrease of CALCIUM level in blood may lead to different disorders. Therefore, measurement of CALCIUM level in blood is an important clinical issue. It is not possible tomeasure CALCIUM in PLASMA samples which contain CALCIUM chelators and we need serumsamples to do so. This research aims to investigate the simple and direct colorimetricmethod for CALCIUM measuring in EDTA treated PLASMA.Materials and Methods: In this experimental study, through adding a patented solution toEDTA treated PLASMA, CALCIUM ions are released from CALCIUM chelators and then reactedwith ortho cresolphthalein complexone (o-CPC) as a coloring reagent and finally productof CALCIUM reaction is measured using microplate reading format. The details ofmethodology are patented on 29.04.2012 in State Organization for Registration of Deedsand Properties with registration number of 74804, and are the exclusive property for theowners. Sensitivity, precision, accuracy and the correlation of PLASMA CALCIUM with serumcalcium level were investigated.Results: Sensitivity of this method was 0.4 mg/dL. Coefficient of variation for intra assaysand inter assay were less than 5% and 6% respectively. According to recovery andparallelism tests, ratio and recovery percent ranged from 90 to 110% and correlationcoefficient in compare with determination of CALCIUM in serum by the same reagent was0.92.Conclusion: This method is capable of measuring CALCIUM in EDTA treated PLASMA and inaddition, it has the required sensitivity, precision and accuracy for common clinical tests.

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    APA: Copy

    Razavi, S.Adeleh. (2015). CALCIUM DETERMINATION IN EDTA TREATED PLASMA BY COLORIMETRIC METHOD AND MICROPLATE READING FORMAT. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 17(2), 0-0. SID. https://sid.ir/paper/703938/en

    Vancouver: Copy

    Razavi S.Adeleh. CALCIUM DETERMINATION IN EDTA TREATED PLASMA BY COLORIMETRIC METHOD AND MICROPLATE READING FORMAT. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2015;17(2):0-0. Available from: https://sid.ir/paper/703938/en

    IEEE: Copy

    S.Adeleh Razavi, “CALCIUM DETERMINATION IN EDTA TREATED PLASMA BY COLORIMETRIC METHOD AND MICROPLATE READING FORMAT,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 17, no. 2, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/703938/en

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