ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SERTOLI CELLS BY FLOW CYTOMETRY: DEVELOPMENT OF PROCEDURE

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LAKPOUR MOHAMMAD REZA; AGHAJANPOUR SAMANEH; KORUJI MORTEZA; SHAHVERDI ABDOLHOSSEIN; SADIGHI GILANI MOHAMMAD ALI; SABBAGHIAN MARJAN; AFLATOONIAN REZA; RAJABIAN NAGHANDAR MAJID

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Journal Paper

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Journal: Journal of Reproduction & Infertility Year:2017 | Volume:18 | Issue:2 Page(s): 213-217

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Title

ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SERTOLI CELLS BY FLOW CYTOMETRY: DEVELOPMENT OF PROCEDURE

Author(s)

Keywords

CHARACTERIZATION

CULTURE

FLOW CYTOMETRY

ISOLATION

SERTOLI CELLS

Abstract

Background: The SERTOLI CELLS in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. These cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis.Given that the SERTOLI CELLS play an essential role in spermatogenesis and the lack of these cells leads to the disruption of spermatogenesis, it is necessary to investigate the behavior and performance of these cells. To achieve this goal, the cells must first be extracted. The aim of this study was to develop a procedure to isolate, CULTURE, and characterize human SERTOLI CELLS.Methods: In order to isolate the SERTOLI CELLS of azoospermia patients who underwent (testicular sperm extraction) TESE surgery, washing up and multi_stage enzyme digestion of single cells, CULTURE on petri dishes impregnated with datura stramonium lectin agglutinin (DSA) were done and then the cells were passaged for several times and isolated. For more purification, FLOW CYTOMETRY method with FSH receptor antibody was used. Immunocytochemistry assays and Elisa test for identification of these cells were employed.Results: The purification method resulted in more than 97% purity. The nature of SERTOLI CELLS was confirmed by morphology evaluation, detecting anti-mullerian hormone in sertoli cell CULTURE media and the presence of FSH receptor on SERTOLI CELLS.Conclusion: This study introduced and applied a method to isolate, CULTURE, and purify human SERTOLI CELLS with high purity which made possible further researches on these cells.

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APA: Copy

LAKPOUR, MOHAMMAD REZA, AGHAJANPOUR, SAMANEH, KORUJI, MORTEZA, SHAHVERDI, ABDOLHOSSEIN, SADIGHI GILANI, MOHAMMAD ALI, SABBAGHIAN, MARJAN, AFLATOONIAN, REZA, & RAJABIAN NAGHANDAR, MAJID. (2017). ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SERTOLI CELLS BY FLOW CYTOMETRY: DEVELOPMENT OF PROCEDURE. JOURNAL OF REPRODUCTION AND INFERTILITY, 18(2), 213-217. SID. https://sid.ir/paper/706545/en

Vancouver: Copy

LAKPOUR MOHAMMAD REZA, AGHAJANPOUR SAMANEH, KORUJI MORTEZA, SHAHVERDI ABDOLHOSSEIN, SADIGHI GILANI MOHAMMAD ALI, SABBAGHIAN MARJAN, AFLATOONIAN REZA, RAJABIAN NAGHANDAR MAJID. ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SERTOLI CELLS BY FLOW CYTOMETRY: DEVELOPMENT OF PROCEDURE. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2017;18(2):213-217. Available from: https://sid.ir/paper/706545/en

IEEE: Copy

MOHAMMAD REZA LAKPOUR, SAMANEH AGHAJANPOUR, MORTEZA KORUJI, ABDOLHOSSEIN SHAHVERDI, MOHAMMAD ALI SADIGHI GILANI, MARJAN SABBAGHIAN, REZA AFLATOONIAN, and MAJID RAJABIAN NAGHANDAR, “ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SERTOLI CELLS BY FLOW CYTOMETRY: DEVELOPMENT OF PROCEDURE,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 18, no. 2, pp. 213–217, 2017, [Online]. Available: https://sid.ir/paper/706545/en

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