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Information Journal Paper

Title

SUCCESSFUL HUMAN SPERMATOGONIAL STEM CELLS HOMING IN RECIPIENT MOUSE TESTIS AFTER IN VITRO TRANSPLANTATION AND ORGAN CULTURE

Pages

  513-520

Abstract

 Objective: In vitro TRANSPLANTATION (IVT) of SPERMATOGONIAl stem cells (SSCs) is one of the most recent methods in TRANSPLANTATION in recent decades. In this study, IVT and SSCs homing on seminiferous tubules of host testis in organ culture have been studied.Materials and Methods: In this experimental study, HUMAN SSCs were isolated and their identities were confirmed by tracking their promyelocytic leukemia zinc finger (PLZF) protein. These cells were transplanted to adult AZOOSPERMIA mouse testes using two methods, namely, IVT andin vivo TRANSPLANTATION as TRANSPLANTATION groups, and testes without TRANSPLANTATION of cells were assigned in the control group. Then histomorphometric, immunohistochemical and molecular studies were done after 2 weeks.Results: After two weeks, histomorphometric studies revealed that the number of subsided SPERMATOGONIAl cells (SCs) and the percentage of tubules with subsided SCs in IVT andin vivo groups were significantly more than those in the control group (P<0.05). Immunohistochemical studies in the TRANSPLANTATION groups confirmed that the PLZF protein was expressed in the cells subsided on the seminiferous tubule. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) demonstrated that the PLZF gene expression was only positive in the TRANSPLANTATION groups, but it was not significantly different between the IVT group and thein vivo group (P>0.05).Conclusion: Testicular TISSUE CULTURE conditions after SSC TRANSPLANTATION can help these cells subside on the seminiferous tubule basement membrane.

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    • Cite

    APA: Copy

    MOHAQIQ, MAHDI, MOVAHEDIN, MANSOUREH, MAZAHERI, ZOHREH, & AMIRJANNATI, NASER. (2019). SUCCESSFUL HUMAN SPERMATOGONIAL STEM CELLS HOMING IN RECIPIENT MOUSE TESTIS AFTER IN VITRO TRANSPLANTATION AND ORGAN CULTURE. CELL JOURNAL (YAKHTEH), 20(4), 513-520. SID. https://sid.ir/paper/706594/en

    Vancouver: Copy

    MOHAQIQ MAHDI, MOVAHEDIN MANSOUREH, MAZAHERI ZOHREH, AMIRJANNATI NASER. SUCCESSFUL HUMAN SPERMATOGONIAL STEM CELLS HOMING IN RECIPIENT MOUSE TESTIS AFTER IN VITRO TRANSPLANTATION AND ORGAN CULTURE. CELL JOURNAL (YAKHTEH)[Internet]. 2019;20(4):513-520. Available from: https://sid.ir/paper/706594/en

    IEEE: Copy

    MAHDI MOHAQIQ, MANSOUREH MOVAHEDIN, ZOHREH MAZAHERI, and NASER AMIRJANNATI, “SUCCESSFUL HUMAN SPERMATOGONIAL STEM CELLS HOMING IN RECIPIENT MOUSE TESTIS AFTER IN VITRO TRANSPLANTATION AND ORGAN CULTURE,” CELL JOURNAL (YAKHTEH), vol. 20, no. 4, pp. 513–520, 2019, [Online]. Available: https://sid.ir/paper/706594/en

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