THE EFFECT OF DIFFERENT CONCENTRATIONS OF METHANOL AND AQUEOUS EXTRACTS OF GARLIC ON OPPORTUNISTIC FUNGI SPOROTHRIX SCHENCKII, CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS

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AYAT ELAHI MOUSAVI S.A.; MEHRABIAN M.; YAGHMAEI B.

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Paper Information

Journal: JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES Year:2009 | Volume:7 | Issue:4 (29) Page(s): 227-234

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Title

THE EFFECT OF DIFFERENT CONCENTRATIONS OF METHANOL AND AQUEOUS EXTRACTS OF GARLIC ON OPPORTUNISTIC FUNGI SPOROTHRIX SCHENCKII, CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS

Author(s)

AYAT ELAHI MOUSAVI S.A. | MEHRABIAN M. | YAGHMAEI B. | Issue Writer Certificate

Keywords

ALLIUM SATIVUMQ3

SPOROTHRIX SCHENCKIIQ3

CANDIDA ALBICANSQ3

CRYPTOCOCCUS NEOFORMANSQ3

Abstract

Background and Objective: The curative properties of garlic in medicine have been known for a long time. However, it was only in the last three decades when garlic properties were seriously investigated confirming its potential as therapeutic medicine. The aim of this study was to measure the MIC of aqueous and methanolic extracts of garlic and to compare them with ketoconazole as positive control.Materials and Methods: In this laboratory study, the fresh smelly bulb of garlic was cleaned, skined, dryed and powdered. Garlic powder was solved in 80% methanol and distilled water. This yellow solution remained in the lab for 4 days. After that the stimmed, filtered and concentrated solution was kept remained inside the pipet in oven for 48h in 50°C to make dry extract. Then methanol and aqueous dilutions (0.625 mg/ml, 1.25 mg/ml 2.5 mg/ml, 5 mg/ml, 10mg/ml and 20 mg/ml) were prepared separately from 200mg powder of the concentrated extracts of garlic. Three strains of dermatophytes were cultured on the media which contained different dilutions.Results: This experimental study showed that the rate of the effect of the aqueous extracts of garlic on SPOROTHRIX SCHENCKII in the minimum dilution (0.625mg/ml) was 8% and in the maximum dilution (20mg/ml) was 100%. This rate in methanol extract on the same fungus was 25% and 100% in lOmg/ml dilution, respectively. The amounts of the effects of extracts on CANDIDA ALBICANS were 17% in the minimum dilution and 100% in 10% dilution. The effect of this plant on CRYPTOCOCCUS NEOFORMANS was more than our expectation, because the amount of MIC in all dilutions was close to its minimum dilution (0.625mg/ml).Conclusion: As the effects of the garlic extracts were the same or even more than ketoconazole, and also regarding the side effects of the synthetic drugs, the use of herbs as useful drugs are necessary.

Cites

THE ANTIMICROBIAL EFFECT OF GARLIC EXTRACT ON STAPHYLOCOCOUS AUREUS AND ESCHERICHIA COLI O157: H7 IN READY TO COOK CHICKEN

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APA: Copy

AYAT ELAHI MOUSAVI, S.A., MEHRABIAN, M., & YAGHMAEI, B.. (2009). THE EFFECT OF DIFFERENT CONCENTRATIONS OF METHANOL AND AQUEOUS EXTRACTS OF GARLIC ON OPPORTUNISTIC FUNGI SPOROTHRIX SCHENCKII, CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 7(4 (29)), 227-234. SID. https://sid.ir/paper/70748/en

Vancouver: Copy

AYAT ELAHI MOUSAVI S.A., MEHRABIAN M., YAGHMAEI B.. THE EFFECT OF DIFFERENT CONCENTRATIONS OF METHANOL AND AQUEOUS EXTRACTS OF GARLIC ON OPPORTUNISTIC FUNGI SPOROTHRIX SCHENCKII, CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2009;7(4 (29)):227-234. Available from: https://sid.ir/paper/70748/en

IEEE: Copy

S.A. AYAT ELAHI MOUSAVI, M. MEHRABIAN, and B. YAGHMAEI, “THE EFFECT OF DIFFERENT CONCENTRATIONS OF METHANOL AND AQUEOUS EXTRACTS OF GARLIC ON OPPORTUNISTIC FUNGI SPOROTHRIX SCHENCKII, CRYPTOCOCCUS NEOFORMANS AND CANDIDA ALBICANS,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 7, no. 4 (29), pp. 227–234, 2009, [Online]. Available: https://sid.ir/paper/70748/en

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