DIFFERENTIATION OF HUMAN SCALP ADIPOSE-DERIVED MESENCHYMAL STEM CELLS INTO MATURE NEURAL CELLS ON ELECTROSPUN NANOFIBROUS SCAFFOLDS FOR NERVE TISSUE ENGINEERING APPLICATIONS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FESHARAKI MEHRAFARIN; RAZAVI SHAHNAZ; GHASEMI MOBARAKEH LALEH; BEHJATI MOHADDESEH; YARAHMADIAN REYHANEH; KAZEMI MOHAMMAD; HEJAZI HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:2 Page(s): 168-176

Download Full-Text

View:

276

Download:

183

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF HUMAN SCALP ADIPOSE-DERIVED MESENCHYMAL STEM CELLS INTO MATURE NEURAL CELLS ON ELECTROSPUN NANOFIBROUS SCAFFOLDS FOR NERVE TISSUE ENGINEERING APPLICATIONS

Author(s)

Keywords

DIFFERENTIATION

NEURON

STEM CELLS

TISSUE ENGINEERING

Abstract

Objective: This study aimed to isolate and culture SADS cells, investigate their neurogenic capacity and evaluate their application for nerve TISSUE ENGINEERING.Materials and Methods: In this experimental study, SADS cells were isolated from human adipose tissue. After 7-day treatment of SADS cells with insulin, indomethacin and isobutylmethylxanthine, neurogenic DIFFERENTIATION of SADS cells was investigated. During this study, Poly (e-caprolactone) (PCL) and PCL/gelatin nanofibrous scaffolds were fabricated using electrospinning and subsequently nanofibrous scaffolds were coated with platelet-rich plasma (PRP). SADS cells were also seeded on nanofibrous scaffolds and neurogentic DIFFERENTIATION of these cells on nanofibers was also evaluated. Effect of PRP on proliferation and DIFFERENTIATION of SADS cells on scaffolds was also studied.Results: Our results showed that after 7-day treatment of SADS cells with insulin, indomethacin and isobutylmethylxanthine, SADS cells expressed markers characteristic of neural cells such as nestin and NEURON specific nuclear protein (NEUN) (as early NEURONal markers) as well as microtubule-associated protein 2 (MAP2) and NEURONal microtubule-associated (TAU) (as mature NEURONal markers) while mature astrocyte maker (GFAP) was not expressed. MTT assay and SEM results showed that incorporation of gelatin and PRP into the structure of nanofibrous scaffolds has a significant positive influence on the bioactivity of scaffolds. Our results also showed neurogentic DIFFERENTIATION of SADS cells on scaffolds.Conclusion: Our results demonstrated that SADS cells have potential to differentiate into early and mature progenitor NEURONs, in vitro. PCL/gelatin/PRP was found to be a promising substrate for proliferation of SADS cells and DIFFERENTIATION of these cells into neural cells which make these scaffolds a candidate for further in vivo experiments and suggest their application for nerve TISSUE ENGINEERING.

Cites

No record.

References

Cite

APA: Copy

FESHARAKI, MEHRAFARIN, RAZAVI, SHAHNAZ, GHASEMI MOBARAKEH, LALEH, BEHJATI, MOHADDESEH, YARAHMADIAN, REYHANEH, KAZEMI, MOHAMMAD, & HEJAZI, HOSSEIN. (2018). DIFFERENTIATION OF HUMAN SCALP ADIPOSE-DERIVED MESENCHYMAL STEM CELLS INTO MATURE NEURAL CELLS ON ELECTROSPUN NANOFIBROUS SCAFFOLDS FOR NERVE TISSUE ENGINEERING APPLICATIONS. CELL JOURNAL (YAKHTEH), 20(2), 168-176. SID. https://sid.ir/paper/708872/en

Vancouver: Copy

FESHARAKI MEHRAFARIN, RAZAVI SHAHNAZ, GHASEMI MOBARAKEH LALEH, BEHJATI MOHADDESEH, YARAHMADIAN REYHANEH, KAZEMI MOHAMMAD, HEJAZI HOSSEIN. DIFFERENTIATION OF HUMAN SCALP ADIPOSE-DERIVED MESENCHYMAL STEM CELLS INTO MATURE NEURAL CELLS ON ELECTROSPUN NANOFIBROUS SCAFFOLDS FOR NERVE TISSUE ENGINEERING APPLICATIONS. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(2):168-176. Available from: https://sid.ir/paper/708872/en

IEEE: Copy

MEHRAFARIN FESHARAKI, SHAHNAZ RAZAVI, LALEH GHASEMI MOBARAKEH, MOHADDESEH BEHJATI, REYHANEH YARAHMADIAN, MOHAMMAD KAZEMI, and HOSSEIN HEJAZI, “DIFFERENTIATION OF HUMAN SCALP ADIPOSE-DERIVED MESENCHYMAL STEM CELLS INTO MATURE NEURAL CELLS ON ELECTROSPUN NANOFIBROUS SCAFFOLDS FOR NERVE TISSUE ENGINEERING APPLICATIONS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 2, pp. 168–176, 2018, [Online]. Available: https://sid.ir/paper/708872/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops