Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018

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Mozafari Haniyeh; MIRKALANTARI SHIVA; SADEGHI KALANI BEHROOZ; AMIRMOZAFARI NOUR

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2021 | Volume:15 | Issue:3 Page(s): 266-272

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Title

Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018

Author(s)

Mozafari Haniyeh | MIRKALANTARI SHIVA | SADEGHI KALANI BEHROOZ | AMIRMOZAFARI NOUR | Issue Writer Certificate

Keywords

Acinetobacter baumannii

Multiplex Polymerase Chain Reaction

Pneumonia

Virulence Factors

Abstract

Background and Aim: Acinetobacter baumannii is considered to be a re-emerging causative agent of nosocomial infections. There is a significant relation between pathogenicity of this bacterium and the numerous Virulence Factors. The purpose of this study was to investigate nine virulence factor genes in A. baumannii isolates derived from hospitalized patients. Materials and Methods: A total of 50 A. baumannii isolates were recovered from patients with Pneumonia in Imam Khomeini Hospital, Tehran, Iran. Following biochemical and microbiological identification of the bacteria, Multiplex PCR was performed for basD, plD, csuA genes, surA, pbpG, bfmR genes, and bap, ompA genes using specific sets of primers which were specifically designed for this study. The espA was identified separately by a Uniplex PCR assay. All amplified DNA fragments were sequenced for the products’ confirmation. Results: Among the 50 clinical isolates of A. baumannii studied, bfmR and pbpG genes were reported in all samples (100%), bap, plD, surA, and csuA genes were collected from 49 samples (98%), 48 (96%) of these isolates had ompA and basD genes, and espA gene was observed in only five isolates (10%). Conclusion: According to this study results, Virulence Factors genes in clinical A. baumannii have a prevalence rate more than 90%. Additionally, the high incidence rate of those genes related to biofilm formation indicates that most clinical strains have the ability to form biofilm structures.

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APA: Copy

Mozafari, Haniyeh, MIRKALANTARI, SHIVA, SADEGHI KALANI, BEHROOZ, & AMIRMOZAFARI, NOUR. (2021). Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 15(3 ), 266-272. SID. https://sid.ir/paper/709716/en

Vancouver: Copy

Mozafari Haniyeh, MIRKALANTARI SHIVA, SADEGHI KALANI BEHROOZ, AMIRMOZAFARI NOUR. Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2021;15(3 ):266-272. Available from: https://sid.ir/paper/709716/en

IEEE: Copy

Haniyeh Mozafari, SHIVA MIRKALANTARI, BEHROOZ SADEGHI KALANI, and NOUR AMIRMOZAFARI, “Prevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 15, no. 3 , pp. 266–272, 2021, [Online]. Available: https://sid.ir/paper/709716/en

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