ALGINATE MICROCAPSULES AS NUTRIENT SUPPLIERS: AN IN VITRO STUDY

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KHOSHZABAN AHAD; KEYHANVAR PEYMAN; DELRISH ELHAM; NAJAFI FARHOOD; HEIDARI KESHEL SAEED; WATANABE IKUYA; VALANEZHAD ALIREZA; JAFARZADEH KASHI TAHEREH SADAT

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:1 Page(s): 25-30

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Title

ALGINATE MICROCAPSULES AS NUTRIENT SUPPLIERS: AN IN VITRO STUDY

Author(s)

Keywords

ALGINATE

CELL CULTURE

CELL VIABILITY

GROWTH MEDIA

MICROCAPSULE

Abstract

Objective: ALGINATE, known as a group of anionic polysaccharides extracted from seaweeds, has attracted the attention of researchers because of its biocompatibility and degradability properties. ALGINATE has shown beneficial effects on wound healing as it has similar function as extracellular matrix. ALGINATE MICROCAPSULEs (AM) that are used in tissue engineering as well as Dulbecco’s modiﬁd Eagle’s medium (DMEM) contain nutrients required for CELL VIABILITY. The purpose of this research was introducing AM in medium and nutrient reagent cells and making a comparison with control group cells that have been normally cultured in long term.Materials and Methods: In this experimental study, AM were shaped in distilled water, it was dropped at 5 mL/hours through a flat 25G5/8 sterile needle into a crosslinking bath containing 0.1 M calcium chloride to produce calcium ALGINATE microspheres. Then, the size of MICROCAPSULEs (300-350 mm) were confirmed by Scanning Electron Microscopy (SEM) images after the filtration for selection of the best size. Next, DMEM was injected into AM. Afterward, adipose derived mesenchymal stem cells (ADSCs) and Ringer’s serum were added. Then, MTT and DAPI assays were used for CELL VIABILITY and nucleus staining, respectively. Also, morphology of MICROCAPSULEs was determined under invert microscopy.Results: Evaluation of the cells performed for spatial media/microcapsules at the volume of 40 ml, showed ADSCs after 1-day CELL CULTURE. Also, MTT assay results showed a significant difference in the viability of sustained-release media injected to MICROCAPSULEs (P<0.05). DAPI staining revealed living cells on the MICROCAPSULEs after 1 to 7-day CELL CULTURE.Conclusion: According to the results, AM had a positive effect on CELL VIABILITY in scaffolds and tissue engineering and provide nutrients needed in cell therapy.

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APA: Copy

KHOSHZABAN, AHAD, KEYHANVAR, PEYMAN, DELRISH, ELHAM, NAJAFI, FARHOOD, HEIDARI KESHEL, SAEED, WATANABE, IKUYA, VALANEZHAD, ALIREZA, & JAFARZADEH KASHI, TAHEREH SADAT. (2018). ALGINATE MICROCAPSULES AS NUTRIENT SUPPLIERS: AN IN VITRO STUDY. CELL JOURNAL (YAKHTEH), 20(1), 25-30. SID. https://sid.ir/paper/710953/en

Vancouver: Copy

KHOSHZABAN AHAD, KEYHANVAR PEYMAN, DELRISH ELHAM, NAJAFI FARHOOD, HEIDARI KESHEL SAEED, WATANABE IKUYA, VALANEZHAD ALIREZA, JAFARZADEH KASHI TAHEREH SADAT. ALGINATE MICROCAPSULES AS NUTRIENT SUPPLIERS: AN IN VITRO STUDY. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(1):25-30. Available from: https://sid.ir/paper/710953/en

IEEE: Copy

AHAD KHOSHZABAN, PEYMAN KEYHANVAR, ELHAM DELRISH, FARHOOD NAJAFI, SAEED HEIDARI KESHEL, IKUYA WATANABE, ALIREZA VALANEZHAD, and TAHEREH SADAT JAFARZADEH KASHI, “ALGINATE MICROCAPSULES AS NUTRIENT SUPPLIERS: AN IN VITRO STUDY,” CELL JOURNAL (YAKHTEH), vol. 20, no. 1, pp. 25–30, 2018, [Online]. Available: https://sid.ir/paper/710953/en

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