EVALUATING THE EFFECT OF MELATONIN ON HAS2, AND PGR EXPRESSION, AS WELL AS CUMULUS EXPANSION, AND FERTILITY POTENTIAL IN MICE

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EZZATI MARYAM; ROSHANGAR LEILA; SOLEIMANI RAD JAFAR; Karimian Nahid

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:1 Page(s): 108-112

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Title

EVALUATING THE EFFECT OF MELATONIN ON HAS2, AND PGR EXPRESSION, AS WELL AS CUMULUS EXPANSION, AND FERTILITY POTENTIAL IN MICE

Author(s)

EZZATI MARYAM | ROSHANGAR LEILA | SOLEIMANI RAD JAFAR | Karimian Nahid | Issue Writer Certificate

Keywords

HYALURONAN SYNTHASE-2

MELATONIN

MOUSE OOCYTE

PROGESTERONE RECEPTOR

Abstract

Objective: Infertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is MELATONIN. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if MELATONIN could regulate HYALURONAN SYNTHASE-2 (HAS2) and PROGESTERONE RECEPTOR (PGR) expressions in the cumulus cells of mice oocytes and provide anin vitro fertilization (IVF) approach.Materials and Methods: In this experimental study, for this purpose, 30 adult female mice and 15 adult male mice were used. The female mice were superovulated using 10 U of pregnant mare serum gonadotropin (PMSG) and 24 hours later, 10 U of human chorionic gonadotropin (hCG) were injected. Next, cumulus oocyte complexes (COCs) were collected from the oviducts of the female mice by using a matrix-flushing method. The cumulus cells were cultured with MELATONIN 10 μM for 6 hours and for real-time reverse transcription-polymerase chain reaction (RT-PCR) was used for evaluation ofHAS2 and PGR expression levels. The fertilization rate was evaluated through IVF. All the data were analyzed using a t test.Results: The results of this study showed that HAS2 and PGR expressions in the cumulus cells of the mice receiving MELATONIN increased in comparison to the control groups. Also, IVF results revealed an enhancement in fertilization rate in the experimental groups compared to the control groups.Conclusion: To improve the oocyte quality and provide new approaches for infertility treatment, administration of MELATONIN as an antioxidant, showed promising results. Thus, it is concluded that fertility outcomes can be improved by MELATONIN it enhances PGR.

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APA: Copy

EZZATI, MARYAM, ROSHANGAR, LEILA, SOLEIMANI RAD, JAFAR, & Karimian, Nahid. (2018). EVALUATING THE EFFECT OF MELATONIN ON HAS2, AND PGR EXPRESSION, AS WELL AS CUMULUS EXPANSION, AND FERTILITY POTENTIAL IN MICE. CELL JOURNAL (YAKHTEH), 20(1), 108-112. SID. https://sid.ir/paper/710962/en

Vancouver: Copy

EZZATI MARYAM, ROSHANGAR LEILA, SOLEIMANI RAD JAFAR, Karimian Nahid. EVALUATING THE EFFECT OF MELATONIN ON HAS2, AND PGR EXPRESSION, AS WELL AS CUMULUS EXPANSION, AND FERTILITY POTENTIAL IN MICE. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(1):108-112. Available from: https://sid.ir/paper/710962/en

IEEE: Copy

MARYAM EZZATI, LEILA ROSHANGAR, JAFAR SOLEIMANI RAD, and Nahid Karimian, “EVALUATING THE EFFECT OF MELATONIN ON HAS2, AND PGR EXPRESSION, AS WELL AS CUMULUS EXPANSION, AND FERTILITY POTENTIAL IN MICE,” CELL JOURNAL (YAKHTEH), vol. 20, no. 1, pp. 108–112, 2018, [Online]. Available: https://sid.ir/paper/710962/en

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