THE EFFECTS OF IN VITRO MATURATION TECHNIQUE ON THE EXPRESSION OF GENES INVOLVED IN EMBRYONIC GENOME ACTIVATION OF HUMAN EMBRYOS

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DORFESHAN PARVIN; GHAFFARI NOVIN MAREFAT; SALEHI MOHAMMAD; MASTERI FARAHANI REZA; FADAEI FATHABADI FATEMEH; SEHATTI RONAK

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:1 Page(s): 90-97

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Title

THE EFFECTS OF IN VITRO MATURATION TECHNIQUE ON THE EXPRESSION OF GENES INVOLVED IN EMBRYONIC GENOME ACTIVATION OF HUMAN EMBRYOS

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Keywords

EMBRYONIC DEVELOPMENT

INTRACYTOPLASMIC SPERM INJECTION

IN VITRO MATURATION

OVARIAN STIMULATION

Abstract

Objective: IN VITRO MATURATION technique (IVM) is shown to have an effect on full maturation of immature oocytes and the subsequent embryo development. Embryonic genome activation (EGA) is considered as a crucial and the first process after fertilization. EGA failure leads to embryo arrest and possible implantation failure. This study aimed to determine the role of IVM in EGA-related genes expression in human embryo originated from immature oocytes and recovered from women receiving gonadotrophin treatment for assisted reproduction.Materials and Methods: In this experimental study, germinal vesicle (GV) oocytes were cultured in vitro. After INTRACYTOPLASMIC SPERM INJECTION of the oocytes, fertilization, cleavage and embryo quality score were assessedin vitroand in vivo. After 3-4 days, a single blastomere was biopsied from the embryos and then frozen. Afterwards, the expression of EGA-related genes in embryos was assayed using quantitative reverse transcriptase-polymerase chain reaction (PCR).Results: The in vitro study showed reduced quality of embryos. No significant difference was found between embryo quality scores for the two groups (P=0.754). Thein vitro group exhibited a relatively reduced expression of the EGArelated genes, when compared to thein vivo group (all of them showed P=0.0001).Conclusion: Although displaying the normal morphology, the IVM process appeared to have a negative influence on developmental gene expression levels of human preimplanted embryos. Based on our results, the embryo normal morphology cannot be considered as an ideal scale for the successful growth of embryo at implantation and downstream processes.

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APA: Copy

DORFESHAN, PARVIN, GHAFFARI, NOVIN MAREFAT, SALEHI, MOHAMMAD, MASTERI FARAHANI, REZA, FADAEI FATHABADI, FATEMEH, & SEHATTI, RONAK. (2018). THE EFFECTS OF IN VITRO MATURATION TECHNIQUE ON THE EXPRESSION OF GENES INVOLVED IN EMBRYONIC GENOME ACTIVATION OF HUMAN EMBRYOS. CELL JOURNAL (YAKHTEH), 20(1), 90-97. SID. https://sid.ir/paper/711607/en

Vancouver: Copy

DORFESHAN PARVIN, GHAFFARI NOVIN MAREFAT, SALEHI MOHAMMAD, MASTERI FARAHANI REZA, FADAEI FATHABADI FATEMEH, SEHATTI RONAK. THE EFFECTS OF IN VITRO MATURATION TECHNIQUE ON THE EXPRESSION OF GENES INVOLVED IN EMBRYONIC GENOME ACTIVATION OF HUMAN EMBRYOS. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(1):90-97. Available from: https://sid.ir/paper/711607/en

IEEE: Copy

PARVIN DORFESHAN, NOVIN MAREFAT GHAFFARI, MOHAMMAD SALEHI, REZA MASTERI FARAHANI, FATEMEH FADAEI FATHABADI, and RONAK SEHATTI, “THE EFFECTS OF IN VITRO MATURATION TECHNIQUE ON THE EXPRESSION OF GENES INVOLVED IN EMBRYONIC GENOME ACTIVATION OF HUMAN EMBRYOS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 1, pp. 90–97, 2018, [Online]. Available: https://sid.ir/paper/711607/en

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