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Title

IN-VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL STEM CELLS TO INSULIN-PRODUCING CELLS

Pages

  69-82

Abstract

 Background & Objective: Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability to differentiate into INSULIN-Producing Cells (IPCs). In this study, Human Umbilical Mesenchymal Stem Cells (HUMSCS) were induced to differentiate into pancreatic b-like cells.Materials & Methods: The samples were collected after cesarean section delivery at Hafez hospital. HUMSCS were cultured in sterile condition, in three steps for 20 days in DMEM-F12, Retinoic Acid (RA), Epidermal Growth Factor (EGF), exendin-4, Fetal Bovine Serum (FBS), and antibiotic. Then, they were differentiated into IPC. DTZ staining employed for determining the presence of INSULIN and Reveres Transcription- Polymerase Chain Reaction (RT-PCR) was done for identifying of gene expression including INSULIN, PDX1, and NGN3. The INSULIN concentration was also evaluated by Immunoradiometric assay.Results: HUMSCS gradually changed from fibroblast-shaped cells to epithelial-like cells and eventually to IPC under special conditions. RT-PCR experiments revealed that these cells expressed INSULIN, PDX1, and NGN3 genes. The cells became red color when stained with DTZ and the INSULIN secretion was confirmed.Conclusion: HUMSCS have the ability to differentiate into islet-like cells in vitro and may be a new potential source for cell transplantation in diabetes treatment.

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    APA: Copy

    NEKOHI MADOEI, S.M., AZARPIRA, N., SADEGHI, L., & KAMALI FAR, S.. (2015). IN-VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL STEM CELLS TO INSULIN-PRODUCING CELLS. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 5(1 ), 69-82. SID. https://sid.ir/paper/713183/en

    Vancouver: Copy

    NEKOHI MADOEI S.M., AZARPIRA N., SADEGHI L., KAMALI FAR S.. IN-VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL STEM CELLS TO INSULIN-PRODUCING CELLS. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2015;5(1 ):69-82. Available from: https://sid.ir/paper/713183/en

    IEEE: Copy

    S.M. NEKOHI MADOEI, N. AZARPIRA, L. SADEGHI, and S. KAMALI FAR, “IN-VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL STEM CELLS TO INSULIN-PRODUCING CELLS,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 5, no. 1 , pp. 69–82, 2015, [Online]. Available: https://sid.ir/paper/713183/en

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