MIR-302B-3P PROMOTES SELF-RENEWAL PROPERTIES IN LEUKEMIA INHIBITORY FACTOR-WITHDRAWN EMBRYONIC STEM CELLS

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MORADI SHARIF; BRAUN THOMAS; BAHARVAND HOSSEIN

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:1 Page(s): 61-72

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Title

MIR-302B-3P PROMOTES SELF-RENEWAL PROPERTIES IN LEUKEMIA INHIBITORY FACTOR-WITHDRAWN EMBRYONIC STEM CELLS

Author(s)

MORADI SHARIF | BRAUN THOMAS | BAHARVAND HOSSEIN | Issue Writer Certificate

Keywords

DIFFERENTIATION

EMBRYONIC STEM CELLS

MICRORNA

MIR-302

SELF-RENEWAL

Abstract

Objective: EMBRYONIC STEM CELLS (ESCs) are regulated by a gene regulatory circuitry composed of transcription factors, signaling pathways, metabolic mediators, and non-coding RNAs (ncRNAs). MICRORNAs (miRNAs) are short ncRNAs which play crucial roles in ESCs. Here, we explored the impact of MIR-302b-3p on ESC SELF-RENEWAL in the absence of leukemia inhibitory factor (LIF).Materials and Methods: In this experimental study, ESCs were cultured in the presence of 15% fetal bovine serum (FBS) and induced to differentiate by LIF removal. MIR-302b-3p overexpression was performed by transient transfection of mature miRNA mimics. Cell cycle profiling was done using propidium iodide (PI) staining followed by flow cytometry.miRNA expression was quantified using a MIR-302b-3p-specific TaqMan assay. Data were analyzed using t test, and a P<0.05 was considered statistically significant.Results: We observed that MIR-302b-3p promoted the viability of both wild-type and LIF-withdrawn ESCs. It also increased ESC clonogenicity and alkaline phosphatase (AP) activity. The defective cell cycling of LIF-deprived ESCs was completely rescued by MIR-302b-3p delivery. Moreover, MIR-302b-3p inhibited the increased cell death rate induced by LIF removal.Conclusion: MIR-302b-3p, as a pluripotency-associated miRNA, promotes diverse features of ESC SELF-RENEWAL in the absence of extrinsic LIF signals.

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APA: Copy

MORADI, SHARIF, BRAUN, THOMAS, & BAHARVAND, HOSSEIN. (2018). MIR-302B-3P PROMOTES SELF-RENEWAL PROPERTIES IN LEUKEMIA INHIBITORY FACTOR-WITHDRAWN EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH), 20(1), 61-72. SID. https://sid.ir/paper/713627/en

Vancouver: Copy

MORADI SHARIF, BRAUN THOMAS, BAHARVAND HOSSEIN. MIR-302B-3P PROMOTES SELF-RENEWAL PROPERTIES IN LEUKEMIA INHIBITORY FACTOR-WITHDRAWN EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(1):61-72. Available from: https://sid.ir/paper/713627/en

IEEE: Copy

SHARIF MORADI, THOMAS BRAUN, and HOSSEIN BAHARVAND, “MIR-302B-3P PROMOTES SELF-RENEWAL PROPERTIES IN LEUKEMIA INHIBITORY FACTOR-WITHDRAWN EMBRYONIC STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 1, pp. 61–72, 2018, [Online]. Available: https://sid.ir/paper/713627/en

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