مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

NAPHTOYL-GLYCYL-GLYCYL-GLYCINE: A NEW SUBSTRATE FOR ANGIOTENSIN CONVERTING ENZYME (ACE) ASSAY USING HPLC

Pages

  76-80

Abstract

 Background: Several in vitro ASSAYs are used to determine ANGIOTENSIN CONVERTING ENZYME (ACE) activity. The purpose of the present investigation, was developing a practical and extraction-free chromatographic method to determine ACE activity.Methods: The method relies on UV-detection of NAPHTHOYL-GLYCINE (NG), which is resulted from enzymatic hydrolysis of the synthetic SUBSTRATE, NAPHTHOYL-GLYCYL-GLYCYL-GLYCINE (NGGG), applying a reverse phase chromatographic separation. In this regard, experimental conditions for the ASSAY such as Enzyme/Substrate (E/S) ratio and incubation time were optimized. Chromatographic separation was performed on a reverse phase C18 column (250×4.6 mm), using a mobile phase consisting of acetonitrile/water (20: 80, v/v), pH=5.0 with a flow rate of 2.0 mL/min and a detection wavelength of 280 nm.Results: The optimized Enzyme/Substrate (E/S) ratio and incubation time were 10 mU/nmol and 35 min respectively. The results indicated that the calibration curve was linear (r2=0.994) and the average recovery (n=6) of NG was 99.5±1.3% (mean±RSD).Conclusion: In this study, we introduced a method which is an efficient approach to determine ACE activity due to its sensitive, accurate, and reliable performance with great repeatability.

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    APA: Copy

    PAPAN, MOHAMMAD KAZEM, SHARIFI, NIUSHA, ZEEB, MOHSEN, MOZAFFARI, SHAHLA, HOSSEINI SEDEH, SEYED REZA, & AMANLOU, MASSOUD. (2016). NAPHTOYL-GLYCYL-GLYCYL-GLYCINE: A NEW SUBSTRATE FOR ANGIOTENSIN CONVERTING ENZYME (ACE) ASSAY USING HPLC. PHARMACEUTICAL SCIENCES, 22(2), 76-80. SID. https://sid.ir/paper/714634/en

    Vancouver: Copy

    PAPAN MOHAMMAD KAZEM, SHARIFI NIUSHA, ZEEB MOHSEN, MOZAFFARI SHAHLA, HOSSEINI SEDEH SEYED REZA, AMANLOU MASSOUD. NAPHTOYL-GLYCYL-GLYCYL-GLYCINE: A NEW SUBSTRATE FOR ANGIOTENSIN CONVERTING ENZYME (ACE) ASSAY USING HPLC. PHARMACEUTICAL SCIENCES[Internet]. 2016;22(2):76-80. Available from: https://sid.ir/paper/714634/en

    IEEE: Copy

    MOHAMMAD KAZEM PAPAN, NIUSHA SHARIFI, MOHSEN ZEEB, SHAHLA MOZAFFARI, SEYED REZA HOSSEINI SEDEH, and MASSOUD AMANLOU, “NAPHTOYL-GLYCYL-GLYCYL-GLYCINE: A NEW SUBSTRATE FOR ANGIOTENSIN CONVERTING ENZYME (ACE) ASSAY USING HPLC,” PHARMACEUTICAL SCIENCES, vol. 22, no. 2, pp. 76–80, 2016, [Online]. Available: https://sid.ir/paper/714634/en

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