مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

377
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

149
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PROMOTER METHYLATION STATUS OF SURVIVAL-RELATED GENES IN MOLT- 4 CELLS CO-CULTURED WITH BONE MARROW MESENCHYMAL STEM CELLS UNDER HYPOXIC CONDITIONS

Pages

  188-194

Abstract

 Objective: DNA METHYLATION is a well-studied epigenetic mechanism that is a potent arm of the gene expression controlling machinery. Since the hypoxic situation and the various cells of bone marrow microenvironment, e.g. MESENCHYMAL STEM CELLs, play a role in the in vivo and in vitro biology of leukemic cells, we decided to study the effects of hypoxia and MESENCHYMAL STEM CELLs (MSCs) on the promoter methylation pattern of BAX and BCL2 genes.Materials and Methods: In this experimental study, the co-culture of MOLT-4 cells with MSCs and treatment with CoCl2 was done during 6, 12, and 24 hour periods. Total DNA was extracted using commercial DNA extraction kits, and sodium bisulfite (SBS) treatment was performed on the extracted DNA. Methylation specific polymerase chain reaction (MSP) was used to evaluate the methylation status of the selected genes’ PROMOTER REGIONS.Results: The BAX and BCL2 promoters of untreated MOLT-4 cells were in partial methylated and fully unmethylated states, respectively. After incubating the cancer cells with CoCl2 and MSCs, the MSP results after 6, 12, and 24 hours were the same as untreated MOLT-4 cells. In other words, the exposure of MOLT-4 cells to the hypoxia-mimicry agent and MSCs in various modes and different time frames showed that these factors have exerted no change on the methylation signature of the studied fragments from the promoter region of the mentioned genes.Conclusion: Hypoxia and MSCs actually have no notable effect on the methylation status of the promoters of BAX and BCL2 in the specifically studied regions. DNA METHYLATION is probably not the main process by which MSCs and CoCl2 induced hypoxia regulate the expression of these genes. Finally, we are still far from discovering the exact functional mechanisms of gene expression directors, but these investigations can provide new insights into this field for upcoming studies.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    AHANI NAHAYATI, MILAD, Solali, Saeed, SHAMSASENJAN, KARIM, MOVASSAGHPOUR AKBARI, ALI AKBAR, TALEBI, MEHDI, ZADI HEYDARABAD, MILAD, BAHARAGHDAM, SINA, & FARSHDOUSTI HAGH, MAJID. (2018). PROMOTER METHYLATION STATUS OF SURVIVAL-RELATED GENES IN MOLT- 4 CELLS CO-CULTURED WITH BONE MARROW MESENCHYMAL STEM CELLS UNDER HYPOXIC CONDITIONS. CELL JOURNAL (YAKHTEH), 20(2), 188-194. SID. https://sid.ir/paper/715407/en

    Vancouver: Copy

    AHANI NAHAYATI MILAD, Solali Saeed, SHAMSASENJAN KARIM, MOVASSAGHPOUR AKBARI ALI AKBAR, TALEBI MEHDI, ZADI HEYDARABAD MILAD, BAHARAGHDAM SINA, FARSHDOUSTI HAGH MAJID. PROMOTER METHYLATION STATUS OF SURVIVAL-RELATED GENES IN MOLT- 4 CELLS CO-CULTURED WITH BONE MARROW MESENCHYMAL STEM CELLS UNDER HYPOXIC CONDITIONS. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(2):188-194. Available from: https://sid.ir/paper/715407/en

    IEEE: Copy

    MILAD AHANI NAHAYATI, Saeed Solali, KARIM SHAMSASENJAN, ALI AKBAR MOVASSAGHPOUR AKBARI, MEHDI TALEBI, MILAD ZADI HEYDARABAD, SINA BAHARAGHDAM, and MAJID FARSHDOUSTI HAGH, “PROMOTER METHYLATION STATUS OF SURVIVAL-RELATED GENES IN MOLT- 4 CELLS CO-CULTURED WITH BONE MARROW MESENCHYMAL STEM CELLS UNDER HYPOXIC CONDITIONS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 2, pp. 188–194, 2018, [Online]. Available: https://sid.ir/paper/715407/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button