ESTABLISHMENT OF A PROTOCOL FOR IN VITRO CULTURE OF CARDIOGENIC MESODERMAL CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

VAHDAT SADAF; Pahlavan Sara; AGHDAMI NASSER; BAKHSHANDEH BEHNAZ; BAHARVAND HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2019 | Volume:20 | Issue:4 Page(s): 496-504

Download Full-Text

View:

291

Download:

305

Cites:

Information Journal Paper

Title

ESTABLISHMENT OF A PROTOCOL FOR IN VITRO CULTURE OF CARDIOGENIC MESODERMAL CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS

Author(s)

Keywords

CARDIOMYOCYTES

CELL DIFFERENTIATION

MATRIGEL

MULTIPOTENT STEM CELLS

Abstract

Objective: Cardiovascular progenitor cells (CPCs) are introduced as one of the promising cell sources for preclinical studies and regenerative medicine. One of the earliest type of CPCs is cardiogenic mesoderm cells (CMCs), which have the capability to generate all types of cardiac lineage derivatives. In order to benefit from CMCs, development of an efficient culture strategy is required. We aim to explore an optimized culture condition that uses human embryonic stem cell (hESC) -derived CMCs.Materials and Methods: In this experimental study, hESCs were expanded and induced toward cardiac lineage in a suspension culture. Mesoderm posterior 1-positive (MESP1+) CMCs were subjected to four different culture conditions: i. Suspension culture of CMC spheroids, ii. Adherent culture of CMC spheroids, iii. Adherent culture of single CMCs using gelatin, and iv. Adherent culture of single CMCs using MATRIGEL.Results: Although, we observed no substantial changes in the percentage of MESP1+ cells in different culture conditions, there were significantly higher viability and total cell numbers in CMCs cultured on MATRIGEL (condition iv) compared to the other groups. CMCs cultivated on MATRIGEL maintained their progenitor cell signature, which included the tendency for cardiogenic differentiation.Conclusion: These results showed the efficacy of an adherent culture on MATRIGEL for hESC-derived CMCs, which would facilitate their use for future applications.

Cites

No record.

References

No record.

Cite

APA: Copy

VAHDAT, SADAF, Pahlavan, Sara, AGHDAMI, NASSER, BAKHSHANDEH, BEHNAZ, & BAHARVAND, HOSSEIN. (2019). ESTABLISHMENT OF A PROTOCOL FOR IN VITRO CULTURE OF CARDIOGENIC MESODERMAL CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH), 20(4), 496-504. SID. https://sid.ir/paper/715508/en

Vancouver: Copy

VAHDAT SADAF, Pahlavan Sara, AGHDAMI NASSER, BAKHSHANDEH BEHNAZ, BAHARVAND HOSSEIN. ESTABLISHMENT OF A PROTOCOL FOR IN VITRO CULTURE OF CARDIOGENIC MESODERMAL CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2019;20(4):496-504. Available from: https://sid.ir/paper/715508/en

IEEE: Copy

SADAF VAHDAT, Sara Pahlavan, NASSER AGHDAMI, BEHNAZ BAKHSHANDEH, and HOSSEIN BAHARVAND, “ESTABLISHMENT OF A PROTOCOL FOR IN VITRO CULTURE OF CARDIOGENIC MESODERMAL CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 4, pp. 496–504, 2019, [Online]. Available: https://sid.ir/paper/715508/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops