In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Khalilifar Mohammad Ali; BAGHABAN ESLAMINEJAD MOHAMADREZA; GHASEMZADEH MOHAMMAD; HOSSEINI SAMANEH; BAHARVAND HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2019 | Volume:21 | Issue:2 Page(s): 150-160

Download Full-Text

View:

297

Download:

261

Cites:

Information Journal Paper

Title

In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair

Author(s)

Keywords

Articular Cartilage

Mesenchymal Stem Cells

Rabbit

Transplantation

Abstract

Objective: Systematic studies indicate a growing number of clinical studies that use Mesenchymal Stem Cells (MSCs) for the treatment of cartilage lesions. The current experimental and preclinical study aims to comparatively evaluate the potential of MSCs from a variety of tissues for the treatment of cartilage defect in Rabbit’ s knee which has not previously been reported. Materials and Methods: In this experimental study, MSCs isolated from bone marrow (BMMSCs), adipose (AMSCs), and ears (EMSCs) of Rabbits and expanded under in vitro culture. The growth rate and differentiation ability of MSCs into chondrocyte and the formation of cartilage pellet were investigated by drawing the growth curve and real-time polymerase chain reaction (RT-PCR), respectively. Then, the critical cartilage defect was created on the Articular Cartilage (AC) of the Rabbit distal femur, and MSCs in collagen carrier were transplanted. The studied groups were as the control (only defect), sham (defect with scaffold), BMMSCs in the scaffold, EMSCs in the scaffold, and EMSCs in the scaffold with cartilage pellets. Histological and the gene expression analysis were performed following the Transplantation. Results: Based on our comparative in vitro investigation, AMSCs possessed the highest growth rate, as well as the lowest chondrogenic differentiation potential. In this context, MSCs of the ear showed a significantly higher growth rate and cartilage differentiation potential than those of bone marrow tissue (P<0. 05). According to our in vivo assessments, BMMSC-and EMSC-seeded scaffolds efficiently improved the cartilage defect 4 weeks post-Transplantation, while no improvement was observed in the group contained the cartilage pellets. Conclusion: It seems that the ear contains MSCs that promote cartilage regeneration as much as the conventional MSCs from the bone marrow. Considering a high proliferation rate and easy harvesting of MSCs of the ear, this finding could be of value for the regenerative medicine.

Cites

The effect of spraying the suspension containing adipose derived mesenchymal stem cells on diabetic wound healing and collagen synthesis in skin tissue in an animal model

References

No record.

Cite

APA: Copy

Khalilifar, Mohammad Ali, BAGHABAN ESLAMINEJAD, MOHAMADREZA, GHASEMZADEH, MOHAMMAD, HOSSEINI, SAMANEH, & BAHARVAND, HOSSEIN. (2019). In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair. CELL JOURNAL (YAKHTEH), 21(2), 150-160. SID. https://sid.ir/paper/717522/en

Vancouver: Copy

Khalilifar Mohammad Ali, BAGHABAN ESLAMINEJAD MOHAMADREZA, GHASEMZADEH MOHAMMAD, HOSSEINI SAMANEH, BAHARVAND HOSSEIN. In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair. CELL JOURNAL (YAKHTEH)[Internet]. 2019;21(2):150-160. Available from: https://sid.ir/paper/717522/en

IEEE: Copy

Mohammad Ali Khalilifar, MOHAMADREZA BAGHABAN ESLAMINEJAD, MOHAMMAD GHASEMZADEH, SAMANEH HOSSEINI, and HOSSEIN BAHARVAND, “In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair,” CELL JOURNAL (YAKHTEH), vol. 21, no. 2, pp. 150–160, 2019, [Online]. Available: https://sid.ir/paper/717522/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops