Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer

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HOMAEI SHANDIZ FATEMEH; FANI AZAR; SHAKERI SEPIDEH; SHEIKHI MARYAM; Ramezani Farkhani Abouzar; SHAJIEI AREZOO; AYATOLLAHI HOSSEIN

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Journal: IRANIAN JOURNAL OF PATHOLOGY (IJP) Year:2017 | Volume:12 | Issue:1 Page(s): 67-73

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Title

Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer

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Keywords

Breast Cancer

HER-2/neu

Gene Quantification

Quantitative Real-Time PCR

Abstract

Background: Breast Cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in Breast Cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence. The amplification of HER-2/neu gene and synthesis of the protein are reported in 10%-34% of Breast Cancer cases associated with tumor size, advanced tumor stage, high-grade tumor, young age at diagnosis, absence of steroid hormone receptor, and lymph node involvement. Methods: Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) methods are options to evaluate HER-2 expression. The current study aimed at identifying the correlation between FISH and real-time polymerase chain reaction (PCR) in measuring HER-2 expression. Results: The study investigated the performance of the real-time PCR as measured against FISH method in IHC +2 borderline cases. In a total of 120 IHC 2+ samples, 58. 3% were negative and 41. 6% positive for HER-2 gene, confirmed by FISH as a gold standard method. The real-time PCR ratio was <1. 8 for a majority (82. 8%) of the tumor samples with unamplified HER-2 gene by FISH as a gold standard assay. Conclusion: Despite the fact that real-time PCR is a promising method to evaluate HER-2 over expression and a supplementary array to FISH, according to the results of the present study it cannot be utilized instead of gold standard techniques; therefore, additional studies should be carried out to appraise the value of this method to evaluate HER-2 over expression.

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APA: Copy

HOMAEI SHANDIZ, FATEMEH, FANI, AZAR, SHAKERI, SEPIDEH, SHEIKHI, MARYAM, Ramezani Farkhani, Abouzar, SHAJIEI, AREZOO, & AYATOLLAHI, HOSSEIN. (2017). Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer. IRANIAN JOURNAL OF PATHOLOGY (IJP), 12(1), 67-73. SID. https://sid.ir/paper/717687/en

Vancouver: Copy

HOMAEI SHANDIZ FATEMEH, FANI AZAR, SHAKERI SEPIDEH, SHEIKHI MARYAM, Ramezani Farkhani Abouzar, SHAJIEI AREZOO, AYATOLLAHI HOSSEIN. Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer. IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2017;12(1):67-73. Available from: https://sid.ir/paper/717687/en

IEEE: Copy

FATEMEH HOMAEI SHANDIZ, AZAR FANI, SEPIDEH SHAKERI, MARYAM SHEIKHI, Abouzar Ramezani Farkhani, AREZOO SHAJIEI, and HOSSEIN AYATOLLAHI, “Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer,” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 12, no. 1, pp. 67–73, 2017, [Online]. Available: https://sid.ir/paper/717687/en

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