Isolation of Mucorales from biological environment and identification of Rhizopus among the isolates using PCRRFLP

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MADANI MAHBOOBEH; ZIA MOHAMMAD ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahrekord University of Medical Sciences Year:2019 | Volume:21 | Issue:2 Page(s): 98-103

Download Full-Text

View:

221

Download:

213

Cites:

Information Journal Paper

Title

Isolation of Mucorales from biological environment and identification of Rhizopus among the isolates using PCRRFLP

Author(s)

MADANI MAHBOOBEH | ZIA MOHAMMAD ALI | Issue Writer Certificate

Keywords

Mucorales

Rhizopus

PCR-RFLP

Isolation

Environment

Abstract

Background and aims: Mucorales are fungi belonging to the category of Zygomycetes, found much in nature. Culture-based methods for clinical samples are often negative, difficult and time-consuming and mainly identify isolates to the genus level, and sometimes only as Mucorales. Therefore, applying fast and accurate diagnosis methods such as molecular approaches seems necessary. This study aims at isolating Mucorales for determination of Rhizopus genus between the isolates using molecular methods. Methods: In this descriptive observational study, a total of 500 samples were collected from air and different surfaces and inoculated on Sabouraud Dextrose Agar supplemented with chloramphenicol. Then, the fungi belonging to Mucorales were identified and their pure culture was provided. DNA extraction was done using extraction kit and the chloroform method. After amplification, the samples belonging to Mucorales were identified by observing 830 bp bands. For enzymatic digestion, enzyme BmgB1 was applied for identification of Rhizopus species by formation of 593 and 235 bp segments. Results: One hundred pure colonies belonging to Mucorales were identified using molecular methods and after enzymatic digestion, 21 isolates were determined as Rhizopus species. The sequencing of PCR products and macroscopic and microscopic studies confirmed the existence of R. stolonifera, R. oryzae and R. caespitosus in the samples. Conclusion: Generally, developing a reliable method for determining Zygomycete species can be a useful tool for better understanding of the epidemiology of mucoromycosis.

Cites

No record.

References

No record.

Cite

APA: Copy

MADANI, MAHBOOBEH, & ZIA, MOHAMMAD ALI. (2019). Isolation of Mucorales from biological environment and identification of Rhizopus among the isolates using PCRRFLP. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 21(2), 98-103. SID. https://sid.ir/paper/717809/en

Vancouver: Copy

MADANI MAHBOOBEH, ZIA MOHAMMAD ALI. Isolation of Mucorales from biological environment and identification of Rhizopus among the isolates using PCRRFLP. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;21(2):98-103. Available from: https://sid.ir/paper/717809/en

IEEE: Copy

MAHBOOBEH MADANI, and MOHAMMAD ALI ZIA, “Isolation of Mucorales from biological environment and identification of Rhizopus among the isolates using PCRRFLP,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 2, pp. 98–103, 2019, [Online]. Available: https://sid.ir/paper/717809/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops