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Information Journal Paper

Title

Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis

Pages

  281-289

Abstract

 Objective: During the cultivation of Spermatogonial Stem Cells (SSCs) and their conversion into embryonic stem-like (ES-like) cells, transitional ES-like colonies and epiblast-like cells were observable. In the present experimental study, we aimed to analyze the efficiency of the multipotency or Pluripotency Potential of ES-like cells, transitional colonies and epiblast-like cells. Materials and Methods: In this experimental study, SSCs were isolated from transgenic octamer-binding transcription factor 4 (Oct4)-green fluorescent protein (GFP)-reporter mice. During cell culture ES-like, transitional and epiblastlike colonies developed spontaneously. The mRNA and protein expression of pluripotency markers were analyzed by Fluidigm real-time polymerase chain reaction (RT-PCR) and immunocytochemistry, respectively. Efficiency to produce chimera mice was evaluated after injection of ES and ES-like cells into blastocysts. Results: Microscopic analyses demonstrated that the expression of Oct4-GFP in ES-like cells was very strong, in epiblast-like cells was not detectable, and was only partial in transitional colonies. Fluidigm RT-PCR showed a higher expression of the germ cell markers Stra-8 and Gpr-125 in ES-like cells and the pluripotency genes Dppa5, Lin28, Klf4, Gdf3 and Tdgf1 in ES-like colonies and embryonic stem cells (ESCs) compared to the epiblast-like and transitional colonies. No significant expression of Oct-4, Nanog, Sox2 and c-Myc was observed in the different groups. We showed a high expression level of Nanog and Klf4 in ES-like, while only a partial expression was observed in transitional colonies. We generated chimeric mice after blastocystic injection from ES and ES-like cells, but not from transitional colonies. We observed that the efficiency to produce chimeric mice in ES cells was more efficient (59%) in comparison to ES-like cells (22%). Conclusion: This new data provides more information on the pluripotency or multipotency potentials of testis-derived ES-like cells in comparison to transitional colonies and epiblast-like cells.

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    Cite

    APA: Copy

    AZIZI, HOSSEIN, Asgari, Behruz, & SKUTELLA, THOMAS. (2019). Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis. CELL JOURNAL (YAKHTEH), 21(3), 281-289. SID. https://sid.ir/paper/718183/en

    Vancouver: Copy

    AZIZI HOSSEIN, Asgari Behruz, SKUTELLA THOMAS. Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis. CELL JOURNAL (YAKHTEH)[Internet]. 2019;21(3):281-289. Available from: https://sid.ir/paper/718183/en

    IEEE: Copy

    HOSSEIN AZIZI, Behruz Asgari, and THOMAS SKUTELLA, “Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis,” CELL JOURNAL (YAKHTEH), vol. 21, no. 3, pp. 281–289, 2019, [Online]. Available: https://sid.ir/paper/718183/en

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