Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAGHIGHAT SOMAYEH; TAVALAEE MARZIYEH; ZAKERI ZAHRA; NOUREDDINI MAHDI; SHAHVERDI ABDOLHOSSEIN; NASR ESFAHANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2019 | Volume:21 | Issue:3 Page(s): 314-321

Download Full-Text

View:

252

Download:

266

Cites:

Information Journal Paper

Title

Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure

Author(s)

Keywords

DNA Fragmentation

Fertilization

Globozoospermia

Male Infertility

Abstract

Objective: Phospholipase C zeta 1 (PLCζ ) is one of the main sperm factor involved in oocyte activation and other factors may assist this factor to induce successful Fertilization. Microinjection of recombinant tr-kit, a truncated form of c-kit receptor, into metaphase II-arrested mouse oocytes initiate egg activation. Considering the potential roles of tr-KIT during spermiogenesis and Fertilization, we aimed to assess expression of tr-KIT in sperm of men with normal and abnormal parameters and also in infertile men with previous failed Fertilization and Globozoospermia. Materials and Methods: This experimental study was conducted from September 2015 to July 2016 on 30 normozoospermic and 20 abnormozoospermic samples for experiment one, and also was carried out on 10 globozoospermic men, 10 men with a history low or failed Fertilization and 13 fertile men for experiment two. Semen parameters and sperm DNA Fragmentation were assessed according to WHO protocol, and TUNEL assay. Sperm tr-KIT was evaluated by flow cytometry, immunostaining and western blot. Results: The results show that tr-KIT mainly was detected in post-acrosomal, equatorial and tail regions. Percentage of tr-KIT-positive spermatozoa in abnormozoospermic men was significantly lower than normozoospermic men. Also significant correlations were observed between sperm tr-KIT with sperm count (r=0. 8, P<0. 001), motility (r=0. 31, P=0. 03) and abnormal morphology (r=-0. 6, P<0. 001). Expression of tr-KIT protein was significantly lower in infertile men with low/ failed Fertilization and Globozoospermia compared to fertile men. The significant correlation was also observed between tr-KIT protein with Fertilization rate (r=-0. 46, P=0. 04). In addition, significant correlations were observed between sperm DNA Fragmentation with Fertilization rate (r=-0. 56, P=0. 019) and tr-KIT protein (r=-0. 38, P=0. 04). Conclusion: tr-KIT may play a direct or indirect role in Fertilization. Therefore, to increase our insight regarding the role of tr-KIT in Fertilization further research is warranted.

Cites

No record.

References

Expression of sperm PLCζ and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion

Cite

APA: Copy

HAGHIGHAT, SOMAYEH, TAVALAEE, MARZIYEH, ZAKERI, ZAHRA, NOUREDDINI, MAHDI, SHAHVERDI, ABDOLHOSSEIN, & NASR ESFAHANI, MOHAMMAD HOSSEIN. (2019). Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure. CELL JOURNAL (YAKHTEH), 21(3), 314-321. SID. https://sid.ir/paper/718867/en

Vancouver: Copy

HAGHIGHAT SOMAYEH, TAVALAEE MARZIYEH, ZAKERI ZAHRA, NOUREDDINI MAHDI, SHAHVERDI ABDOLHOSSEIN, NASR ESFAHANI MOHAMMAD HOSSEIN. Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure. CELL JOURNAL (YAKHTEH)[Internet]. 2019;21(3):314-321. Available from: https://sid.ir/paper/718867/en

IEEE: Copy

SOMAYEH HAGHIGHAT, MARZIYEH TAVALAEE, ZAHRA ZAKERI, MAHDI NOUREDDINI, ABDOLHOSSEIN SHAHVERDI, and MOHAMMAD HOSSEIN NASR ESFAHANI, “Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure,” CELL JOURNAL (YAKHTEH), vol. 21, no. 3, pp. 314–321, 2019, [Online]. Available: https://sid.ir/paper/718867/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops