Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability

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BIDKHORI HAMID REZA; AHMADIANKIA NAGHMEH; MOGHADDAM MATIN MARYAM; HEIRANI TABASI ASIEH; FARSHCHIAN MOEIN; NADERI MESHKIN HOJJAT; Shahriyari Mina; Dastpak Mahtab; BAHRAMI AHMAD REZA

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:1 Page(s): 14-19

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Title

Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability

Author(s)

Keywords

CXCR4

CXCR7

CoCl2

Desferrioxamine

MSC

Chemical treatment

Homing

Valproic acid

Abstract

Objective(s): The limited Homing potential of bone-marrow-derived mesenchymal stem cells (BM-MSC) is the key obstacle in MSC-based therapy. It is believed that chemokines and chemokine receptor interactions play key roles in cellular processes associated with migration. Meanwhile, MSCs express a low level of distinct chemokine receptors and they even lose these receptors on their surface after a few passages which influence their therapeutic applications negatively. This study investigated whether treatment of BM-MSCs with hypoxia-mimicking agents would increase expression of some chemokine receptors and cell migration. Materials and Methods: BM-MSCs were treated at passage 2 for our gene expression profiling. All qPCR experiments were performed by SYBR Green method in CFX-96 Bio-Rad Real-Time PCR. The Boyden chamber assay was utilized to investigate BM-MSC Homing. Results: Possible approaches to increasing the expression level of chemokine receptors by different hypoxia-mimicking agents such as Valproic acid (VPA), CoCl2, and Desferrioxamine (DFX) are described. Results show DFX efficiently up-regulate the CXCR7 and CXCR4 gene expression while VPA increase only the CXCR7 gene expression and no significant change in expression level of CXCR4 and the CXCR7 gene was detectable by CoCl2 treatment. Chemotaxis assay results show that pre-treatment with DFX, VPA, and CoCl2 enhances significantly the migration ability of BM-MSCs compared with the untreated control group and DFX treatment accelerates MSCs Homing significantly with a higher rate than VPA and CoCl2 treatments. Conclusion: Our data supports the notion that pretreatment of MSC with VPA and DFX improves the efficiency of MSC therapy by triggering Homing regulatory signaling pathways.

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APA: Copy

BIDKHORI, HAMID REZA, AHMADIANKIA, NAGHMEH, MOGHADDAM MATIN, MARYAM, HEIRANI TABASI, ASIEH, FARSHCHIAN, MOEIN, NADERI MESHKIN, HOJJAT, Shahriyari, Mina, & Dastpak, Mahtab. (2016). Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(1), 14-19. SID. https://sid.ir/paper/720649/en

Vancouver: Copy

BIDKHORI HAMID REZA, AHMADIANKIA NAGHMEH, MOGHADDAM MATIN MARYAM, HEIRANI TABASI ASIEH, FARSHCHIAN MOEIN, NADERI MESHKIN HOJJAT, Shahriyari Mina, Dastpak Mahtab. Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(1):14-19. Available from: https://sid.ir/paper/720649/en

IEEE: Copy

HAMID REZA BIDKHORI, NAGHMEH AHMADIANKIA, MARYAM MOGHADDAM MATIN, ASIEH HEIRANI TABASI, MOEIN FARSHCHIAN, HOJJAT NADERI MESHKIN, Mina Shahriyari, and Mahtab Dastpak, “Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 1, pp. 14–19, 2016, [Online]. Available: https://sid.ir/paper/720649/en

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