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Information Journal Paper

Title

Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis

Pages

  172-177

Abstract

 The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’ s teeth pulp revascularized with MTA and Propolis. Materials and Methods: 144 mature and immature two rooted dog’ s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: Propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII Factor. Results: Tissue Regeneration was seen in 64. 5% of MTA, 38% of Propolis, and 0% of parafilm group samples. Expression of VEGF and VIII Factor in the Propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII Factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. Conclusion: According to the results of this study, Propolis can induce the expression of VEGF and VIII Factor in infected mature and immature dog’ s teeth and is a suitable biomaterial for the revascularization technique.

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    APA: Copy

    ZAREI, MINA, JAFARIAN, AMIR HOSSEIN, HARANDI, AZADEH, JAVIDI, MARYAM, & GHARECHAHI, MARYAM. (2017). Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 172-177. SID. https://sid.ir/paper/720869/en

    Vancouver: Copy

    ZAREI MINA, JAFARIAN AMIR HOSSEIN, HARANDI AZADEH, JAVIDI MARYAM, GHARECHAHI MARYAM. Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):172-177. Available from: https://sid.ir/paper/720869/en

    IEEE: Copy

    MINA ZAREI, AMIR HOSSEIN JAFARIAN, AZADEH HARANDI, MARYAM JAVIDI, and MARYAM GHARECHAHI, “Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 172–177, 2017, [Online]. Available: https://sid.ir/paper/720869/en

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