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Information Journal Paper

Title

A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies

Pages

  360-367

Abstract

 Detection of circulating Mycobacterium Tuberculosis (M. Tuberculosis) Antigens is promising in Tuberculosis (TB) Diagnosis. However, not a single Antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal Antibodies targeting multiple Antigen markers (multi-target Antibodies) and evaluate their efficacies in TB Diagnosis. Materials and Methods: A fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed. The fusion polyprotein was used as an immunogen to elicit production of multi-target Antibodies. Their reactivities were tested. Then, the multi-target Antibodies and three corresponding Antibodies elicited by each single Antigen (mono-target Antibodies) were evaluated with sandwich Elisa for detecting M. Tuberculosis Antigens. Their diagnostic efficacies for TB were also compared. Results: The polyprotein successfully elicited production of multi-target Antibodies targeting 38kD, ESAT6, and CFP10 as analyzed by Western blotting. When used as coating Antibodies, the multi-target Antibodies were more efficient in capturing the three Antigens than the corresponding mono-target Antibodies. By testing clinical serum, the multi-target Antibodies demonstrated significantly higher sensitivity for clinical TB Diagnosis than all three mono-target Antibodies. Conclusion: The multi-target Antibodies allowed detecting multiple Antigens simultaneously and significantly enhanced TB detection compared to routine mono-target Antibodies. Our study may provide a promising strategy for TB Diagnosis.

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    APA: Copy

    Dai, Zhenhua, Liu, Zhiqiang, Xiu, Bingshui, Yang, Xiqin, ZHAO, PING, Zhang, Xuhui, Duan, Cuimi, Que, Haiping, Zhang, Heqiu, & Feng, Xiaoyan. (2017). A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(4), 360-367. SID. https://sid.ir/paper/720873/en

    Vancouver: Copy

    Dai Zhenhua, Liu Zhiqiang, Xiu Bingshui, Yang Xiqin, ZHAO PING, Zhang Xuhui, Duan Cuimi, Que Haiping, Zhang Heqiu, Feng Xiaoyan. A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(4):360-367. Available from: https://sid.ir/paper/720873/en

    IEEE: Copy

    Zhenhua Dai, Zhiqiang Liu, Bingshui Xiu, Xiqin Yang, PING ZHAO, Xuhui Zhang, Cuimi Duan, Haiping Que, Heqiu Zhang, and Xiaoyan Feng, “A multiple-antigen detection assay for tuberculosis diagnosis based on broadly reactive polyclonal antibodies,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 4, pp. 360–367, 2017, [Online]. Available: https://sid.ir/paper/720873/en

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