Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody

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Ranjibar Farideh; HABIBI ANBOUHI MAHDI; KAZEMI LOMEDASHT FATEMEH; Aghaee Bakhtiyari Seyed Hamid; Alirahimi Ehsan; BEHDANI MAHDI

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:9 Page(s): 884-888

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Title

Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody

Author(s)

Keywords

Bacteriophage

Nanobody

Receptor-mediated genetransfer

Targeted-gene delivery

VEGFR2

Abstract

Objective(s): Filamentous Bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific Nanobody was genetically fused to the capsid gene III of M13 Bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR). Results: Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP. Conclusion: It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches.

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APA: Copy

Ranjibar, Farideh, HABIBI ANBOUHI, MAHDI, KAZEMI LOMEDASHT, FATEMEH, Aghaee Bakhtiyari, Seyed Hamid, Alirahimi, Ehsan, & BEHDANI, MAHDI. (2018). Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(9), 884-888. SID. https://sid.ir/paper/720907/en

Vancouver: Copy

Ranjibar Farideh, HABIBI ANBOUHI MAHDI, KAZEMI LOMEDASHT FATEMEH, Aghaee Bakhtiyari Seyed Hamid, Alirahimi Ehsan, BEHDANI MAHDI. Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(9):884-888. Available from: https://sid.ir/paper/720907/en

IEEE: Copy

Farideh Ranjibar, MAHDI HABIBI ANBOUHI, FATEMEH KAZEMI LOMEDASHT, Seyed Hamid Aghaee Bakhtiyari, Ehsan Alirahimi, and MAHDI BEHDANI, “Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 9, pp. 884–888, 2018, [Online]. Available: https://sid.ir/paper/720907/en

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