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Information Journal Paper

Title

Kalirin-7 plays a neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

Pages

  992-997

Abstract

 Objective(s): The study explored the neuroprotective role of Kalirin-7 (Kal-7) in Neuro-2A cells after oxygen-glucose deprivation and reperfusion (OGD/R) treatment. Materials and Methods: The study used an OGD/R model of mouse Neuro-2A neuroblastoma cells in vitro. Cells were transfected with pCAGGS-Kal-7 to up-regulating kal-7. Then cell proliferation and apoptosis were respectively analyzed by Trypan blue exclusion method and flow cytometry. To examine the involvement of Rac1, cells were treated with Rac1-GTP inhibitor NSC23766 before treatment with OGD/R. Expressions of Bax, Bcl-2, Rac1, and down-stream targets of Rac1 were analyzed by Western blot. Results: Kal-7 significantly decreased OGD/R induced cell apoptosis (P<0. 01), but no significant effects were observed on cell proliferation. Kal-7 increased the expressions of apoptosis-related protein of Bcl-2 and Rac1, but decreased the expression of Bax in Neuro-2A cells stimulated to OGD/R. Rac1 was activated by Kal-7 due to the increased levels of its down-stream targets, p-p38 and p-PAK1. NSC23766 reduced the Anti-apoptotic effect of Kal-7 as the enhanced apoptotic cell rate and increased Bax/Bcl-2 ratio. Conclusion: These findings suggest that the protective effects of Kal-7 against OGD/R injury in Neuro-2A cells were dependent in a Rac1 activation signaling.

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    APA: Copy

    XU, JIAN, Chen, Yanxiu, Wu, Zeyu, Dou, Yihe, Lun, Peng, & Sun, Peng. (2018). Kalirin-7 plays a neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(10), 992-997. SID. https://sid.ir/paper/721474/en

    Vancouver: Copy

    XU JIAN, Chen Yanxiu, Wu Zeyu, Dou Yihe, Lun Peng, Sun Peng. Kalirin-7 plays a neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(10):992-997. Available from: https://sid.ir/paper/721474/en

    IEEE: Copy

    JIAN XU, Yanxiu Chen, Zeyu Wu, Yihe Dou, Peng Lun, and Peng Sun, “Kalirin-7 plays a neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 10, pp. 992–997, 2018, [Online]. Available: https://sid.ir/paper/721474/en

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