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Information Journal Paper

Title

C-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal

Pages

  720-725

Abstract

 Objective(s): To study the C-Kit expression in the gallbladder of cholesterol lithogenic guinea pig model and the effect of Artemisia capillaris Thunb on Interstitial cells of Cajal (ICCs). Materials and Methods: A total of 45 guinea pigs were randomly assigned into three groups: the control group (guinea pigs fed a standard diet, normal group); the model group (guinea pigs fed a cholesterol Gallstone-inducing diet); and the Chinese medicine group (guinea pigs fed the cholesterol Gallstone-inducing diet and treated with A. capillaris through intragastric administration, therapy group). Each group had 15 guinea pigs. The gallbladders of the guinea pigs were harvested after 8 weeks. C-Kit expression was detected using an immunohistochemistry staining, real-time PCR, and Western blot analyses. The effect of A. capillaris on ICCs was evaluated by muscle strip contraction experiments. Results: C-Kit expression significantly decreased in the gallbladder of model group, but increased in the Chinese medicine group. The Contractility of guinea pig gallbladder muscle strip significantly improved in the Chinese medicine group. Conclusion: Our results indicated that A. capillaris improves gallbladder impairment by up-regulating C-Kit expression, and it also can improve the contractile response of in vitro guinea pig gallbladder muscle strips.

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    • Cite

    APA: Copy

    FENG, HUA, WANG, FANG, & Wang, Changmiao. (2016). C-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(7), 720-725. SID. https://sid.ir/paper/721786/en

    Vancouver: Copy

    FENG HUA, WANG FANG, Wang Changmiao. C-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(7):720-725. Available from: https://sid.ir/paper/721786/en

    IEEE: Copy

    HUA FENG, FANG WANG, and Changmiao Wang, “C-Kit expression in the gallbladder of guinea pig with chronic calculous cholecystitis and the effect of Artemisia capillaris Thunb on interstitial cells of Cajal,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 7, pp. 720–725, 2016, [Online]. Available: https://sid.ir/paper/721786/en

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