Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae

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Inapurapu Santhipriya; Kudle Karunakar Rao; Bodiga Sreedhar; Bodiga Vijaya Lakshmi

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Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:1 Page(s): 83-89

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Title

Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae

Author(s)

Inapurapu Santhipriya | Kudle Karunakar Rao | Bodiga Sreedhar | Bodiga Vijaya Lakshmi | Issue Writer Certificate

Keywords

Cisplatin

Mitochondria

Oxidative phosphorylation

Respiration

Rho0

Abstract

To understand the role of Mitochondrial Respiration in Cisplatin sensitivity, we have employed wild-type and Mitochondrial DNA depleted Rho0 yeast cells. Materials and Methods: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against Cisplatin by monitoring growth curves, oxygen consumption, pH changes in cytosol/mitochondrial compartments, reactive oxygen species production and respiratory control ratio. Results: Wild-type yeast grown on glycerol exhibited heightened sensitivity to Cisplatin than yeast grown on glucose. Cisplatin (100 μ M), although significantly reduced the growth of wild-type cells, only slightly altered the growth rate of Rho0 cells. Cisplatin treatment decreased both pHcyt and pHmit to a similar extent without affecting the pH difference. Cisplatin dose-dependently increased the oxidative stress in wild-type, but not in Respiration-deficient Rho0 strain. Cisplatin decreased the respiratory control ratio. Conclusion: These results suggest that Cisplatin toxicity is influenced by the respiratory capacity of the cells and the intracellular oxidative burden. Although Cisplatin per se slightly decreased the Respiration of yeast cells grown in glucose, it did not disturb the Mitochondrial chemiosmotic gradient

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APA: Copy

Inapurapu, Santhipriya, Kudle, Karunakar Rao, Bodiga, Sreedhar, & Bodiga, Vijaya Lakshmi. (2017). Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(1), 83-89. SID. https://sid.ir/paper/721884/en

Vancouver: Copy

Inapurapu Santhipriya, Kudle Karunakar Rao, Bodiga Sreedhar, Bodiga Vijaya Lakshmi. Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(1):83-89. Available from: https://sid.ir/paper/721884/en

IEEE: Copy

Santhipriya Inapurapu, Karunakar Rao Kudle, Sreedhar Bodiga, and Vijaya Lakshmi Bodiga, “Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 1, pp. 83–89, 2017, [Online]. Available: https://sid.ir/paper/721884/en

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