CFP10: mFcγ 2 as a novel tuberculosis vaccine candidate increases immune response in mouse

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Baghani Ali Asghar; SOLEIMANPOUR SAMAN; farsiani hadi; Mosavat Arman; YOUSEFI MASOUD; MESHKAT ZAHRA; REZAEE SEYED ABDOLRAHIM; AMEL JAMEHDAR SAEID; AKBARI EYDGAHI MOHAMMAD REZA; SADEGHIAN HAMID; GHAZVINI KIARASH

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Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:2 Page(s): 122-130

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Title

CFP10: mFcγ 2 as a novel tuberculosis vaccine candidate increases immune response in mouse

Author(s)

Keywords

CFP-10

Fcγ2a

Immunogenicity

Mycobacterium –tuberculosis

Subunit vaccine

Abstract

Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, Immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel Subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZα A expression vector and recombinant vectors (pPICZα A-CFP-10: Fcγ 2a and pPICZα A-CFP-10: His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The Immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ , IL-12, IL-4, IL-17 and TGF-β ) were evaluated. Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10: Fcγ 2a and CFP-10: His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10: His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ 2a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the Immunogenicity of the Subunit vaccine. Further studies, might be able to design and produce a new generation of Subunit vaccines based on the Fc-fused immunogen.

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APA: Copy

Baghani, Ali Asghar, SOLEIMANPOUR, SAMAN, farsiani, hadi, Mosavat, Arman, YOUSEFI, MASOUD, MESHKAT, ZAHRA, REZAEE, SEYED ABDOLRAHIM, AMEL JAMEHDAR, SAEID, AKBARI EYDGAHI, MOHAMMAD REZA, SADEGHIAN, HAMID, & GHAZVINI, KIARASH. (2017). CFP10: mFcγ 2 as a novel tuberculosis vaccine candidate increases immune response in mouse. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 122-130. SID. https://sid.ir/paper/721886/en

Vancouver: Copy

Baghani Ali Asghar, SOLEIMANPOUR SAMAN, farsiani hadi, Mosavat Arman, YOUSEFI MASOUD, MESHKAT ZAHRA, REZAEE SEYED ABDOLRAHIM, AMEL JAMEHDAR SAEID, AKBARI EYDGAHI MOHAMMAD REZA, SADEGHIAN HAMID, GHAZVINI KIARASH. CFP10: mFcγ 2 as a novel tuberculosis vaccine candidate increases immune response in mouse. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):122-130. Available from: https://sid.ir/paper/721886/en

IEEE: Copy

Ali Asghar Baghani, SAMAN SOLEIMANPOUR, hadi farsiani, Arman Mosavat, MASOUD YOUSEFI, ZAHRA MESHKAT, SEYED ABDOLRAHIM REZAEE, SAEID AMEL JAMEHDAR, MOHAMMAD REZA AKBARI EYDGAHI, HAMID SADEGHIAN, and KIARASH GHAZVINI, “CFP10: mFcγ 2 as a novel tuberculosis vaccine candidate increases immune response in mouse,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 122–130, 2017, [Online]. Available: https://sid.ir/paper/721886/en

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