The effect of omega-3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation

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Krizak Jakub; Frimmel Karel; Bernatova Iveta; Navarova Jana; Sotnikova Ruzena; Okruhlicova Ludmila

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:3 Page(s): 290-299

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Title

The effect of omega-3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation

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Keywords

Aorta

Endothelium

Lipopolysaccharide

Occludin

Omega-3 polyunsaturated fatty acids

Abstract

Objective(s): Occludin is essential for proper assembly of tight junctions (TJs) which regulate paracellular endothelial permeability. Omega-3 polyunsaturated fatty acids (Ω-3 PUFA) protect endothelial barrier function against injury. Materials and Methods: We examined anti-inflammatory effect of Ω-3 PUFA intake (30 mg/kg/day for 10 days) on expression and location of Occludin in the Aorta of adult Wistar rats after a single dose of bacterial Lipopolysaccharide (LPS, Escherichia coli, 1 mg/kg). The ultrastructure of TJs after LPS administration was also investigated. We measured plasma levels of C-reactive protein (CRP), Malondialdehyde (MDA) and CD68 expression and determined the total activity of NO synthase (NOS) in the aortic tissue. Results: LPS induced a significant decrease of Occludin expression accompanied by structural alterations of TJs. Levels of CRP, MDA, CD68 and NOS activity were elevated after LPS injection compared to controls indicating presence of moderate inflammation. Ω-3 PUFA supplementation did not affect Occludin expression in treated inflammatory group. However they reduced CRP and MDA concentration and CD68 expression, but conversely, they increased NOS activity compared to inflammatory group. Conclusion: Our results indicate that a single dose of LPS could have a long-term impact on Occludin expression and thus contribute to endothelial barrier dysfunction. 10-day administration of Ω-3 PUFA had partial anti-inflammatory effects on health of rats without any effect on Occludin expression.

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APA: Copy

Krizak, Jakub, Frimmel, Karel, Bernatova, Iveta, Navarova, Jana, Sotnikova, Ruzena, & Okruhlicova, Ludmila. (2016). The effect of omega-3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(3), 290-299. SID. https://sid.ir/paper/722030/en

Vancouver: Copy

Krizak Jakub, Frimmel Karel, Bernatova Iveta, Navarova Jana, Sotnikova Ruzena, Okruhlicova Ludmila. The effect of omega-3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(3):290-299. Available from: https://sid.ir/paper/722030/en

IEEE: Copy

Jakub Krizak, Karel Frimmel, Iveta Bernatova, Jana Navarova, Ruzena Sotnikova, and Ludmila Okruhlicova, “The effect of omega-3 polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 3, pp. 290–299, 2016, [Online]. Available: https://sid.ir/paper/722030/en

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