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Information Journal Paper

Title

The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease

Pages

  1102-1108

Abstract

 Objective(s): In this study, we aimed to investigate the effects of Fas ribozyme/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Anti-Fas ribozyme on the Apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=10 per group). Group A was used as the control. B, C, and D groups were transfected with empty lentivirus, Fas ribozyme/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Anti-Fas ribozyme, and an Fas ribozyme/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Anti-Fas ribozyme mutant, respectively. The expression of Fas mRNA and protein in the T cells were evaluated using qPCR and Western blot, respectively. Flow cytometry was used to evaluate the Apoptosis of CD4＋ T cells and calculate the ratio of CD4+ to CD8+ T cells (CD4＋ /CD8＋ ). Results: Fas ribozyme/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Anti-Fas ribozyme significantly inhibited the expression of Fas in the T cells of COPD mice. In addition, the number of apoptotic CD4＋ T cells and CD4＋ /CD8＋ of the C and D groups were significantly lower and higher than those of group A, respectively (P<0. 05). The apoptotic CD4＋ T cells and CD4＋ CD8＋ of the C group were significantly lower and higher than those of group D, respectively (P<0. 05). Conclusion: Fas ribozyme/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Anti-Fas ribozyme significantly inhibited the expression of Fas, increased CD4+/ CD8+, and inhibited the Apoptosis of T cells in COPD mice.

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    APA: Copy

    Zhuo, Song Ming, Li, Si Cong, Lin, Yong Qun, Yu, Hai Bin, & LI, NA. (2017). The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(10), 1102-1108. SID. https://sid.ir/paper/722033/en

    Vancouver: Copy

    Zhuo Song Ming, Li Si Cong, Lin Yong Qun, Yu Hai Bin, LI NA. The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(10):1102-1108. Available from: https://sid.ir/paper/722033/en

    IEEE: Copy

    Song Ming Zhuo, Si Cong Li, Yong Qun Lin, Hai Bin Yu, and NA LI, “The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 10, pp. 1102–1108, 2017, [Online]. Available: https://sid.ir/paper/722033/en

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