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Information Journal Paper

Title

Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells

Pages

  128-133

Abstract

 Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Materials and Methods: Primary cultured RPE cells were treated with ELF-PEMF (50 Hz) for three days. Using ELISA assay, we evaluated the effects of treatment on RPE cell proliferation and apoptosis. Also, RT-PCR was used to determine the Gene expression of Proangiogenic factors, such as matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α ), VEGFA, cathepsin D, connective tissue growth factor (CTGF), E2F3, tissue inhibitors of metalloproteinases 1 (TIMP-1), and TIMP-2. Results: No noticeable changes were observed in cell proliferation and cell death of ELF-PEMF-exposed RPE cells, while transcript levels of proangiogenic genes (HIF-1α , VEGFA, VEGFR-2, CTGF, cathepsin D, TIMP-1, E2F3, MMP-2, and MMP-9) increased significantly. Conclusion: RPE cells are important for homeostasis of the retina. ELF-PEMF increased the Gene expression of Proangiogenic factors in RPE cells, which highlights concerns about the impact of this treatment on human health.

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    APA: Copy

    OLADNABI, MORTEZA, BAGHERI, ABOUZAR, REZAEI KANAVI, MOZHGAN, AZADMEHR, ABBAS, & KIANMEHR, ANVARSADAT. (2019). Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(2), 128-133. SID. https://sid.ir/paper/722675/en

    Vancouver: Copy

    OLADNABI MORTEZA, BAGHERI ABOUZAR, REZAEI KANAVI MOZHGAN, AZADMEHR ABBAS, KIANMEHR ANVARSADAT. Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(2):128-133. Available from: https://sid.ir/paper/722675/en

    IEEE: Copy

    MORTEZA OLADNABI, ABOUZAR BAGHERI, MOZHGAN REZAEI KANAVI, ABBAS AZADMEHR, and ANVARSADAT KIANMEHR, “Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 2, pp. 128–133, 2019, [Online]. Available: https://sid.ir/paper/722675/en

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