Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice

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Doosti Mohammad; NASSIRI MOHAMMADREZA; NASIRI KHADIJEH; TAHMOORESPUR MOJTABA; ZIBAEE SAEED

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:3 Page(s): 302-309

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Title

Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice

Author(s)

Keywords

Adjuvant

Foot-and-mouth

diseasevirus

Immune response

Interleukin-2

VP1 protein

Abstract

Objective(s): The results of studies on vaccine development for Foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immuno-dominant epitopes have been shown to induce Immune responses. Furthermore, for safety of immunization, access to efficient Adjuvants against FMD virus seems to be critical. Materials and Methods: In this study, we produced epitope recombinant vaccines from the VP1 protein of the FMD virus for serotype O of Iran. Constructs were included polytope (tandem-repeat multipleepitope), polytope coupled with Interleukin-2 (polytope-IL 2) as a molecular Adjuvant and IL-2. Three expression vectors were constructed and expressed in Escherichia coli BL21 (DE3). To evaluate whether these recombinant vaccines induce Immune responses, BALB/c mice were injected with the recombinant vaccines and their Immune responses were compared with a negative control group. The humoral and cellular Immune responses were measured by ELISA. Results: The results showed that IL-2 co-expressed or co-inoculated with Polytope protein enhances the immune effect of multiple epitope recombinant vaccine against FMD virus. The results of total immunoglobulin G (IgG), IgG1, and IgG2a levels and secretion of interferon gamma (IFN-γ ), IL-4 and IL-10 revealed that there were significant differences between negative control group and other injected mice with the recombinant vaccines (P<0. 05). Conclusion: Observations indicated that the epitope recombinant plasmid of the VP1 protein coexpressed or co-inoculated with IL-2 was effective in inducing an enhanced Immune response. Therefore, IL-2 can be recommended as a potential Adjuvant for epitope recombinant vaccine of the VP1 protein from FMD virus.

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References

Design and construction of multiple-epitope recombinant vaccine against foot-and-mouth disease virus type O.

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APA: Copy

Doosti, Mohammad, NASSIRI, MOHAMMADREZA, NASIRI, KHADIJEH, TAHMOORESPUR, MOJTABA, & ZIBAEE, SAEED. (2019). Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(3), 302-309. SID. https://sid.ir/paper/722687/en

Vancouver: Copy

Doosti Mohammad, NASSIRI MOHAMMADREZA, NASIRI KHADIJEH, TAHMOORESPUR MOJTABA, ZIBAEE SAEED. Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(3):302-309. Available from: https://sid.ir/paper/722687/en

IEEE: Copy

Mohammad Doosti, MOHAMMADREZA NASSIRI, KHADIJEH NASIRI, MOJTABA TAHMOORESPUR, and SAEED ZIBAEE, “Effect of IL-2 co-expressed or co-inoculated with immunodominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 3, pp. 302–309, 2019, [Online]. Available: https://sid.ir/paper/722687/en

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