SURVIVABILITY OF PROBIOTIC BACTERIA IN SIMULATED GASTRIC AND INTESTINAL MODEL

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SARABI JAMAB M.; RAHNAMA VOSOUGH P.; KATE SHAMSHIRI M.; KARAZHYAN R.

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Paper Information

Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2017 | Volume:14 | Issue:68 Page(s): 331-340

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Information Journal Paper

Title

SURVIVABILITY OF PROBIOTIC BACTERIA IN SIMULATED GASTRIC AND INTESTINAL MODEL

Author(s)

SARABI JAMAB M. | RAHNAMA VOSOUGH P. | KATE SHAMSHIRI M. | KARAZHYAN R. | Issue Writer Certificate

Keywords

LACTOBACILLUS ACIDOPHILUSQ2

LACTOBACILLUS RHAMNOSUSQ2

BIFIDOBACTERIUM BIFIDUMQ2

SIMULATED GASTRIC AND INTESTINAL MODELQ1

Abstract

In this study survivability of probiotics, Lactobacilus acidophilus, Lactobacilus rhamnosus and BIFIDOBACTERIUM BIFIDUMwas assessed in simulated gastrointestinal tract. The count of probiotic bacteria was done in simulated gastric conditions (pH 1.5 and 2.5) at different incubation times (30, 60, 90 and 120 min) at 37oC. Initial number of live bacteria was 3 × 109 per ml. After incubation in the simulated gastric model (60 min), stomach contents were transferred to simulated intestinal model (pH 7.25) and incubated for mentioned times and the number of viable bacteria was enumerated.Statistical analysis revealed a highly significant difference between the probiotic bacteria, andB.bifidumshowed higher survivability compared to L. acidophilus and L. rhamnosus in SIMULATED GASTRIC AND INTESTINAL MODEL. The count of probiotic bacteria was decreased during every half an hour of incubation times in simulated gastric media. No difference was noticed in declining rate of probiotics in simulated intestinal media except in the first 30 minute which number of bacteria was higher in this period.B. bifidum showed better survivability in both pH of simulated gastric environments. After incubation in simulated intestinal model (pH 7.25, 2hrs) number of survived cells were 2.33 log cfu mL-1 for B. bifidum, 1 log cfu mL-1 for L.acidophilus and 0.65 for L. rhamnosus respectively.

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APA: Copy

SARABI JAMAB, M., RAHNAMA VOSOUGH, P., KATE SHAMSHIRI, M., & KARAZHYAN, R.. (2017). SURVIVABILITY OF PROBIOTIC BACTERIA IN SIMULATED GASTRIC AND INTESTINAL MODEL. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 14(68), 331-340. SID. https://sid.ir/paper/72337/en

Vancouver: Copy

SARABI JAMAB M., RAHNAMA VOSOUGH P., KATE SHAMSHIRI M., KARAZHYAN R.. SURVIVABILITY OF PROBIOTIC BACTERIA IN SIMULATED GASTRIC AND INTESTINAL MODEL. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2017;14(68):331-340. Available from: https://sid.ir/paper/72337/en

IEEE: Copy

M. SARABI JAMAB, P. RAHNAMA VOSOUGH, M. KATE SHAMSHIRI, and R. KARAZHYAN, “SURVIVABILITY OF PROBIOTIC BACTERIA IN SIMULATED GASTRIC AND INTESTINAL MODEL,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 14, no. 68, pp. 331–340, 2017, [Online]. Available: https://sid.ir/paper/72337/en

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