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Information Journal Paper

Title

Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design

Pages

  1073-1084

Abstract

 Objective(s): To address the alarming problem of Methicillin-resistant Staphylococcus aureus (MRSA), herein, a marine Streptomyces capable of producing an anti-MRSA compound has been studied. Materials and Methods: Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound in large scale, a kind of submerged liquid Fermentation was adopted. The antibacterial agent was purified using a silica gel column followed by a semi-preparative HPLC and the isolated metabolite was identified using mass spectrometry, Nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR). Finally, the production process was subjected to a two steps optimization using Plackett-Burman design (PBD) and Response Surface Method (RSM), respectively. In addition, the antitumor activity of the active agent was studied. Results: The purified compound with a molecular weight of 421. 2 was identified as a natural pyrrolederivative. The optimization revealed a significant effect for starch, pH, calcium carbonate and peptone on the production of this anti-MRSA compound and resulted in a 218% increase in the production yield. Conclusion: The isolated pyrrole-derivative showed a remarkable activity against MRSA and also showed some promising anti-tumor activity.

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    Cite

    APA: Copy

    NOROUZI, HAMED, RABBANI KHORASGANI, MOHAMMAD, & Danesh, Abolghasem. (2019). Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(9), 1073-1084. SID. https://sid.ir/paper/724469/en

    Vancouver: Copy

    NOROUZI HAMED, RABBANI KHORASGANI MOHAMMAD, Danesh Abolghasem. Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(9):1073-1084. Available from: https://sid.ir/paper/724469/en

    IEEE: Copy

    HAMED NOROUZI, MOHAMMAD RABBANI KHORASGANI, and Abolghasem Danesh, “Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 9, pp. 1073–1084, 2019, [Online]. Available: https://sid.ir/paper/724469/en

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