Prevalence of β-lactamase genes, class 1 integrons, major virulence factors and clonal relationships of multidrug-resistant Pseudomonas aeruginosa isolated from hospitalized patients in southeast of Iran

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SHARIFI HOSEIN; POULADFAR GHOLAMREZA; SHAKIBAIE MOHAMMAD REZA; POURABBAS BAHMAN; MARDANEH JALAL; MANSOURI SHAHLA

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Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:7 Page(s): 806-812

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Title

Prevalence of β-lactamase genes, class 1 integrons, major virulence factors and clonal relationships of multidrug-resistant Pseudomonas aeruginosa isolated from hospitalized patients in southeast of Iran

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Keywords

Antibiotic resistance

Beta-lactamases

Class 1 integrons

pseudomonas aeruginosa

RAPD-PCR

Virulence factors

Abstract

Objective(s): pseudomonas aeruginosa is one of the most important nosocomial pathogens causing a high rate of mortality among hospitalized patients. Herein, we report the prevalence of Antibiotic resistance genes, Class 1 integrons, major virulence genes and clonal relationship among multidrugresistant (MDR) P. aeruginosa, isolated from four referral hospitals in the southeast of Iran. Materials and Methods: In this study, 208 isolates of P. aeruginosa were collected from four referral hospitals in southeast of Iran. Disk diffusion method was used to determine susceptibility to 13 antibacterial agents. AmpC was detected by phenotypic method and β-lactamase genes, virulence genes and Class 1 integrons were detected by PCR. Clonal relationship of the isolates was determined by RAPD-PCR. Results: All the isolates were susceptible to polymyxin-B and colistin. Overall, 40. 4% of the isolates were MDR, among which resistance to third generation cephalosporins, aminoglycosides, and carbapenems was 47. 5%, 32. 3% and 40%, respectively. None of the isolates was positive for blaNDM-1 genes, while 84. 5% and 4. 8% were positive for the blaIMP-1 and blaVIM, metallo-β-lactamase genes, respectively. Incidence of Class 1 integrons was 95% and AmpC was detected in 33% of the isolates. Prevalence of exoA, exoS, exoU, pilB and nan1 were 98. 8%, 44%, 26%, 8. 3% and 33. 3%, respectively. RAPD profiles identified four large clusters consisting of 77 isolates, and two small clusters and three singletons. Conclusion: : The rate of MDR P. aeruginosa isolates was high in different hospitals in this region. High genetic similarity among MDR isolates suggests cross-acquisition of infection in the region.

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APA: Copy

SHARIFI, HOSEIN, POULADFAR, GHOLAMREZA, SHAKIBAIE, MOHAMMAD REZA, POURABBAS, BAHMAN, MARDANEH, JALAL, & MANSOURI, SHAHLA. (2019). Prevalence of β-lactamase genes, class 1 integrons, major virulence factors and clonal relationships of multidrug-resistant Pseudomonas aeruginosa isolated from hospitalized patients in southeast of Iran. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(7), 806-812. SID. https://sid.ir/paper/725664/en

Vancouver: Copy

SHARIFI HOSEIN, POULADFAR GHOLAMREZA, SHAKIBAIE MOHAMMAD REZA, POURABBAS BAHMAN, MARDANEH JALAL, MANSOURI SHAHLA. Prevalence of β-lactamase genes, class 1 integrons, major virulence factors and clonal relationships of multidrug-resistant Pseudomonas aeruginosa isolated from hospitalized patients in southeast of Iran. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(7):806-812. Available from: https://sid.ir/paper/725664/en

IEEE: Copy

HOSEIN SHARIFI, GHOLAMREZA POULADFAR, MOHAMMAD REZA SHAKIBAIE, BAHMAN POURABBAS, JALAL MARDANEH, and SHAHLA MANSOURI, “Prevalence of β-lactamase genes, class 1 integrons, major virulence factors and clonal relationships of multidrug-resistant Pseudomonas aeruginosa isolated from hospitalized patients in southeast of Iran,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 7, pp. 806–812, 2019, [Online]. Available: https://sid.ir/paper/725664/en

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