مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells

Pages

  705-711

Abstract

 Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced Apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and Apoptosis were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Caspase-3 and-9 activities were determined by a colorimetric assay. The roles of ClC-2 in glutamate-induced Apoptosis were examined by using ClC-2 complementary deoxyribonucleic acid (cDNA) and small inference ribonucleic acid (RNA) transfection technology. Results: Overexpression of ClC-2 in RGC-5 cells significantly decreased glutamate-induced Apoptosis and increased cell viability, whereas silencing of ClC-2 with short hairpin (sh) RNA produced opposite effects. ClC-2 overexpression increased the expression of Bcl-2, decreased the expression of Bax, and decreased caspase-3 and-9 activation in RGC-5 cells treated with glutamate, but silencing of ClC-2 produced opposite effects. Conclusion: Our data suggest that ClC-2 Chloride channels might play a protective role in glutamate-induced Apoptosis in Retinal ganglion cells via the mitochondria-dependent Apoptosis pathway.

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    APA: Copy

    Hong, Sen, Ma, Ling Jun, Zhou, Hong Yan, LU, Jiao, ZHAO, JING, & Zheng, Ya Juan. (2016). Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(7), 705-711. SID. https://sid.ir/paper/726681/en

    Vancouver: Copy

    Hong Sen, Ma Ling Jun, Zhou Hong Yan, LU Jiao, ZHAO JING, Zheng Ya Juan. Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(7):705-711. Available from: https://sid.ir/paper/726681/en

    IEEE: Copy

    Sen Hong, Ling Jun Ma, Hong Yan Zhou, Jiao LU, JING ZHAO, and Ya Juan Zheng, “Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 7, pp. 705–711, 2016, [Online]. Available: https://sid.ir/paper/726681/en

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