MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES

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KESHAVARZI SOMAYEH; SALEHI MOHAMMAD; FARIFTEH NOBIJARI FATTANEH; HOSSEINI TAHER; HOSSEINI SARA; GHAZIFARD ALALEH; GHAFFARI NOVIN MAREFAT; FALLAH OMRANI VAHID; NOUROZIAN MOHSEN; HOSSEINI AHMAD

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:2 Page(s): 244-249

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Title

MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES

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Keywords

GLUTATHIONE

IN VITRO OOCYTE MATURATION

MELATONIN

REACTIVE OXYGEN SPECIES

Abstract

Objective: We evaluated the effect of MELATONIN, as a potent antioxidant agent, on GLUTATHIONE (GSH) and REACTIVE OXYGEN SPECIES (ROS) levels, as well as histone H3 lysine 9 (H3K9), and H4 lysine 12 (H4K12) acetylation when added to oocytes culture medium.Materials and Methods: In this experimental study, two in vitro and in vivo groups were used. In the in vitro group, cumulus oocyte complexes (COCs) from the ovaries of B6D2F1 mice were cultured in maturation medium containing two doses of MELATONIN (10-9 and 10-6 M) and without MELATONIN [control group treated with dimethyl sulfoxide (DMSO)] for 22-24 hour. The cumulus expansion and nuclear status were monitored by an inverted microscope. Next, COCs were isolated from the oviducts of superovulated mice and studied as the in vivo group. In in vitro and in vivo matured oocytes, GSH and ROS levels were assessed by monochlorobimane (MCB) and 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, respectively. Changes in histone acetylation were examined by immunofluorescent staining with specific antibodies against acetylated H3K9 and H4K12. Results: The H4K12 acetylation and ROS levels were significantly higher in the oocytes matured in the in vitro group compared to the in vivo group (P<0.05). Furthermore, GLUTATHIONE levels in the in vitro group were considerably lower than that of the in vivo group (P<0.05). MELATONIN at the concentration of 10-6 M had the most substantial effect on nuclear maturation and histone acetylation as well as GLUTATHIONE and ROS levels in the in vitro group (P<0.05).Conclusion: Exogenous MELATONIN improves the competence of mouse oocytes during in vitro maturation (IVM).

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APA: Copy

KESHAVARZI, SOMAYEH, SALEHI, MOHAMMAD, FARIFTEH NOBIJARI, FATTANEH, HOSSEINI, TAHER, HOSSEINI, SARA, GHAZIFARD, ALALEH, GHAFFARI, NOVIN MAREFAT, FALLAH OMRANI, VAHID, NOUROZIAN, MOHSEN, & HOSSEINI, AHMAD. (2018). MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES. CELL JOURNAL (YAKHTEH), 20(2), 244-249. SID. https://sid.ir/paper/727636/en

Vancouver: Copy

KESHAVARZI SOMAYEH, SALEHI MOHAMMAD, FARIFTEH NOBIJARI FATTANEH, HOSSEINI TAHER, HOSSEINI SARA, GHAZIFARD ALALEH, GHAFFARI NOVIN MAREFAT, FALLAH OMRANI VAHID, NOUROZIAN MOHSEN, HOSSEINI AHMAD. MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(2):244-249. Available from: https://sid.ir/paper/727636/en

IEEE: Copy

SOMAYEH KESHAVARZI, MOHAMMAD SALEHI, FATTANEH FARIFTEH NOBIJARI, TAHER HOSSEINI, SARA HOSSEINI, ALALEH GHAZIFARD, NOVIN MAREFAT GHAFFARI, VAHID FALLAH OMRANI, MOHSEN NOUROZIAN, and AHMAD HOSSEINI, “MELATONIN MODIFIES HISTONE ACETYLATION DURING IN VITRO MATURATION OF MOUSE OOCYTES,” CELL JOURNAL (YAKHTEH), vol. 20, no. 2, pp. 244–249, 2018, [Online]. Available: https://sid.ir/paper/727636/en

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