THE STUDY OF CORRELATIONS BETWEEN B-LACTAMASE AN)) PROTEIN A IN STAPHYLOCOCCUS AUREUS

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KASRA KERMANSHAHI R.; KEYVANI AMINEH H.; GOL ALIPOUR M.

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Paper Information

Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2000 | Volume:2 | Issue:2 (6) Page(s): 16-22

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Information Journal Paper

Title

THE STUDY OF CORRELATIONS BETWEEN B-LACTAMASE AN)) PROTEIN A IN STAPHYLOCOCCUS AUREUS

Author(s)

KASRA KERMANSHAHI R. | KEYVANI AMINEH H. | GOL ALIPOUR M. | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUROUSQ2

B-LACTAMASEQ2

PROTEIN AQ4

ANTIBIOTICQ3

VIRULENCEQ3

Abstract

OBJECTIVE: STAPHYLOCOCCUS AUROUS is the type species and pathogen of human. There is some factors that determines the VIRULENCE of bacteria, but PROTEIN A and B-LACTAMASE are the most important factors. We studied the correlations between two factors of VIRULENCE (B-lactamase and PROTEIN A) in this microorganism.METHODS: We studied the resistance to B-LACTAMASE sensible to B- Lactamase as penicillin G (Pen G), Cefazolin (CeZ), cefalotine (Cfn), resistant to B-LACTAMASE as methicillin (Met) and Oxacillin (Oxa) with disk diffusion method. M.I.C.S. and B-LACTAMASE production detected by broth dilution and acidometric methods. Purification of S.P.A performed on these samples with ion exchange chromatography method (DEAE-Cellulose). Cell wall lysis was performed with heat extraction method.FINDINGS: This results showed that resistance to drugs was 96%(Pen G), 83%(CeZ), 86%(Cfn) and 73% (Oxa). Purification performed on these samples with method (DEAE cellulose) have a sharp dense band in electrophoresis (SDS-PAGE) with a molecular weight of about 42 kd. CONCLUSION: After studding relationships between existence of S.P.A. and B-LACTAMASE, we determined that 93.7% of strains with B-LACTAMASEs had S.P.A. but the strains without B-LACTAMASE only 50% had S.P.A. The result with methicillin resistant strains is most representative because in these kinds of strains there are not any strains with S.P.A

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