Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms

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SALEHZADEH ALI; ZAMANI HOJJATOLAH; Keshtkar Langeroudi Maedeh; MIRZAIE AMIR

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:12 Page(s): 1325-1330

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Title

Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms

Author(s)

SALEHZADEH ALI | ZAMANI HOJJATOLAH | Keshtkar Langeroudi Maedeh | MIRZAIE AMIR | Issue Writer Certificate

Keywords

Adhesion molecules Biofilm Coagulase RFLP S

aureus Spa typing

Abstract

Objective(s): Staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. Biofilm formation is regarded as an important factor in the establishment of S. aureus infection. The contribution of the genetic background of S. aureus to biofilm formation is poorly understood. The aim of the present work was to genotype S. aureus strains associated to biofilm based on the coagulase and protein A genes and to evaluate the association between the genetic background and the biofilm forming ability of clinical S. aureus isolates. Materials and Methods: A total number of 100 S. aureus were isolated from nosocomial infections and biofilm formation capability was investigated using phenotypic assay and molecular detection of biofilm associated genes. The strains were genotyped based on coagulase (coa) and protein A (spa) gene polymorphisms using restriction fragments length polymorphism-polymerase chain reaction (RFLP-PCR). Results: RFLP-PCR of coa gene generated two types and three subtypes. Amplification of spa gene resulted in two banding patterns and their restriction digestion generated three subtypes. The combined coa and spa RFLP patterns generated nine genotypes (G1-G9). The genotypes G4 and G1 were the most prevalent (32. 1% and 24. 3%, respectively). Conclusion: High clonal diversity of S. aureus strains able to produce biofilm was observed. Biofilm formation correlates with the spa and coa clonal lineage in our population and testing for multiple gene polymorphisms could be employed for local epidemiologic purposes.

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APA: Copy

SALEHZADEH, ALI, ZAMANI, HOJJATOLAH, Keshtkar Langeroudi, Maedeh, & MIRZAIE, AMIR. (2016). Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(12), 1325-1330. SID. https://sid.ir/paper/730483/en

Vancouver: Copy

SALEHZADEH ALI, ZAMANI HOJJATOLAH, Keshtkar Langeroudi Maedeh, MIRZAIE AMIR. Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(12):1325-1330. Available from: https://sid.ir/paper/730483/en

IEEE: Copy

ALI SALEHZADEH, HOJJATOLAH ZAMANI, Maedeh Keshtkar Langeroudi, and AMIR MIRZAIE, “Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 12, pp. 1325–1330, 2016, [Online]. Available: https://sid.ir/paper/730483/en

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