EVALUATION OF ANTIMICROBIAL ACTIVITY OF LACTOFERRIN AGAINST P.AERUGINOSA AND E.COLI GROWTH

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SHARBAFI R.; MORADIAN F.; RAFEIE A.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2016 | Volume:18 | Issue:7 Page(s): 19-23

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Title

EVALUATION OF ANTIMICROBIAL ACTIVITY OF LACTOFERRIN AGAINST P.AERUGINOSA AND E.COLI GROWTH

Author(s)

SHARBAFI R. | MORADIAN F. | RAFEIE A. | Issue Writer Certificate

Keywords

ANTIMICROBIAL ACTIVITYQ2

E.COLIQ2

LACTOFERRINQ2

PSEUDOMONAS AEROGINOSAQ2

Abstract

BACKGROUND AND OBJECTIVE: LACTOFERRIN (LF) is an iron-binding glycoprotein that involves a diverse range of biological activities. LACTOFERRIN is a major component of milk and is present in exocrine secretions such as tears, salvia, bile, and neutrophil granules. LACTOFERRIN has more potent antimicrobial activities against a wide range of gram negative and positive bacteria as well as antivirus activities. The purpose of this study is to evaluate the effect of this protein on P.aeruginosa growth in patients with burns that show drug resistance.METHODS: In this study, antibacterial activity of LACTOFERRIN has been scrutinized after isolation and purification of bovine colostrum against PSEUDOMONAS AEROGINOSA. Bacteria samples were isolated from scald patients (Shahid Zare Hospital); then microbial activity was confirmed with biochemical tests like oxidase, catalase and growth on TSI medium. Four concentrations 400, 500, 600 and 700 mg/ml of LACTOFERRIN were assayed. Pseudomonas colonies counted and compared with negative control (without LACTOFERRIN) as well as E.COLI (DH5α) as positive control was considered. FINDINGS: Our results showed that 400mg/ml concentration of LACTOFERRIN has the least inhibitory effect with 35% and 29% growth inhibitory and 700mg/ml concentration of LACTOFERRIN has the highest inhibitory effect with 86% and 66% on Pseudomonas and E.COLI, respectively. CONCLUSION: Our result showed that all of LACTOFERRIN concentrations have inhibitory activity which in 700μg/ml has the highest inhibition against PSEUDOMONAS AEROGINOSA and also E.COLI.

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References

ISOLATION AND PURIFICATION OF BOVINE LACTOFERRIN

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APA: Copy

SHARBAFI, R., MORADIAN, F., & RAFEIE, A.. (2016). EVALUATION OF ANTIMICROBIAL ACTIVITY OF LACTOFERRIN AGAINST P.AERUGINOSA AND E.COLI GROWTH. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 18(7), 19-23. SID. https://sid.ir/paper/73196/en

Vancouver: Copy

SHARBAFI R., MORADIAN F., RAFEIE A.. EVALUATION OF ANTIMICROBIAL ACTIVITY OF LACTOFERRIN AGAINST P.AERUGINOSA AND E.COLI GROWTH. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2016;18(7):19-23. Available from: https://sid.ir/paper/73196/en

IEEE: Copy

R. SHARBAFI, F. MORADIAN, and A. RAFEIE, “EVALUATION OF ANTIMICROBIAL ACTIVITY OF LACTOFERRIN AGAINST P.AERUGINOSA AND E.COLI GROWTH,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 18, no. 7, pp. 19–23, 2016, [Online]. Available: https://sid.ir/paper/73196/en

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