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Information Journal Paper

Title

Hypericin-photodynamic therapy inhibits proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A

Pages

  130-137

Abstract

 Objective(s): To elucidate the effects and potential mechanisms of hypericin-photodynamic therapy (HYP-PDT) for treating the human Rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) MH7A cell-line. Materials and Methods: MH7A cells were subjected to HYP-PDT intervention and Apoptosis was evaluated via MTT, nuclear staining, and flowcytometry analyses. Intracellular Reactive oxygen species (ROS) were measured with the fluorescent probe 2’ 7’-dichlorofluorescein diacetate (DCFH-DA). To verify the effects of HYP on apoptotic and Nuclear factor kappa-B (NF-κ B) pathways, caspase-8, 9, poly-ADP-ribose polymerase (PARP), phosphorylated (p)-NF-κ B p65, NF-κ B p65 and p-Iκ Bα protein expressions were quantified with Western blot. Quantitative real-time PCR was used to assay NF-κ B p65 mRNA. Results: HYP-PDT inhibited MH7A cell viability and induced Apoptosis in a dose-dependent manner. Meanwhile, intracellular ROS levels increased significantly after HYP-PDT treatment. Furthermore, the expression of cleaved caspase-9 and PARP was increased by HYP-PDT treatment, with a concurrent decline in NF-κ B. Conclusion: HYP-PDT induces Apoptosis in MH7A cells, at least partially, via generation of ROS, regulation of the apoptotic pathway and suppression of the NF-κ B pathway. These findings suggest that HYP-PDT may be a potential treatment for RA.

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    APA: Copy

    Zhang, Kun, Gao, Shan, Guo, JiaYi, Guo Hua, Guo Hua, Chen, Zhe, Li, Feng, Zhu, Xiao Lei, Wen, YongBing, & Guo, YanXing. (2018). Hypericin-photodynamic therapy inhibits proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(2), 130-137. SID. https://sid.ir/paper/733802/en

    Vancouver: Copy

    Zhang Kun, Gao Shan, Guo JiaYi, Guo Hua Guo Hua, Chen Zhe, Li Feng, Zhu Xiao Lei, Wen YongBing, Guo YanXing. Hypericin-photodynamic therapy inhibits proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(2):130-137. Available from: https://sid.ir/paper/733802/en

    IEEE: Copy

    Kun Zhang, Shan Gao, JiaYi Guo, Guo Hua Guo Hua, Zhe Chen, Feng Li, Xiao Lei Zhu, YongBing Wen, and YanXing Guo, “Hypericin-photodynamic therapy inhibits proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 2, pp. 130–137, 2018, [Online]. Available: https://sid.ir/paper/733802/en

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