Comparison of the efficacy of piascledine and transforming growth factor β 1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

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HASHEMIBENI BATOOL; VALIANI ALI; Esmaeli Mojtaba; KAZEMI MOHAMMAD; ALIAKBARI MARYAM; GOLSHAN IRANPOUR FARHAD

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Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:2 Page(s): 212-218

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Title

Comparison of the efficacy of piascledine and transforming growth factor β 1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Author(s)

Keywords

Chondrogenesis

Piascledine

Stem cells

Tissue engineering

Transforming growth-factor beta 1

Abstract

Objective(s): The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β 1 on adipose-derived Stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β 1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method. Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<0. 05). Real-time PCR evaluation revealed that the expression of type II collagen was higher in TGF-β 1groups, but the expression of aggrecan was higher in TGF-β 1 alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β 1 in fibrin scaffold. Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds.

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APA: Copy

HASHEMIBENI, BATOOL, VALIANI, ALI, Esmaeli, Mojtaba, KAZEMI, MOHAMMAD, ALIAKBARI, MARYAM, & GOLSHAN IRANPOUR, FARHAD. (2018). Comparison of the efficacy of piascledine and transforming growth factor β 1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(2), 212-218. SID. https://sid.ir/paper/733803/en

Vancouver: Copy

HASHEMIBENI BATOOL, VALIANI ALI, Esmaeli Mojtaba, KAZEMI MOHAMMAD, ALIAKBARI MARYAM, GOLSHAN IRANPOUR FARHAD. Comparison of the efficacy of piascledine and transforming growth factor β 1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(2):212-218. Available from: https://sid.ir/paper/733803/en

IEEE: Copy

BATOOL HASHEMIBENI, ALI VALIANI, Mojtaba Esmaeli, MOHAMMAD KAZEMI, MARYAM ALIAKBARI, and FARHAD GOLSHAN IRANPOUR, “Comparison of the efficacy of piascledine and transforming growth factor β 1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 2, pp. 212–218, 2018, [Online]. Available: https://sid.ir/paper/733803/en

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