Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

275
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

187
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice

Pages

  350-357

Abstract

 Objective: Autograft transplantation of vitrified cortical Ovarian Tissue is an acceptable clinical technique for fertility preservation in women. Xenograft transplantation into animal models could be useful for evaluating the safety of human vitrified Ovarian Tissue. This study targeted to evaluate impact of Vitrification on expression of the genes associated with folliculogenesis after xenograft transplantation of human vitrified Ovarian Tissue to γ-irradiated mice. Materials and Methods: In this experimental study, ovarian biopsies were gathered from six transsexual persons. The cortical section of ovarian biopsies was separated and chopped into small pieces. These pieces were randomly divided into vitrified and non-vitrified groups. In each group some pieces were considered as non-transplanted tissues and the others were transplanted to γ-irradiated female National Medical Research Institute (NMRI) mice. Before and after two weeks of xenograft transplantation, histological assessment and evaluation of the expression of folliculogenesisassociated genes (FIGLA, GDF-9, KL and FSHR) were performed in both vitrified and non-vitrified groups. Results: Percentage of the normal follicles and expression of the all examined genes from transplanted and nontransplanted tissue were similar in both vitrified and non-vitrified groups (P>0. 05). After transplantation, the normal follicle rate was significantly decreased and among the folliculogenesis-associated genes, expression of GDF-9 gene was significantly increased, rather than before transplantation in vitrified and non-vitrified tissues (P<0. 05). Conclusion: The Vitrification method using dimethyl solphoxide and ethylene glycol (EG) had no remarkable effect on the normal follicular rate and expression of folliculogenesis-associated genes after two weeks human Ovarian Tissue xenografting. In addition, transplantation process can cause a significant decrease in normal follicular rate and expression of GDF-9 gene.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    SHAMS MOFARAHE, ZAHRA, GHAFFARI NOVIN, MAREFAT, & SALEHNIA, MOJDEH. (2020). Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice. CELL JOURNAL (YAKHTEH), 22(3), 350-357. SID. https://sid.ir/paper/734044/en

    Vancouver: Copy

    SHAMS MOFARAHE ZAHRA, GHAFFARI NOVIN MAREFAT, SALEHNIA MOJDEH. Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(3):350-357. Available from: https://sid.ir/paper/734044/en

    IEEE: Copy

    ZAHRA SHAMS MOFARAHE, MAREFAT GHAFFARI NOVIN, and MOJDEH SALEHNIA, “Folliculogenesis-Associated Genes Expression in Human Vitrified Ovarian Tissue after Xenotransplantation in γ-Irradiated Mice,” CELL JOURNAL (YAKHTEH), vol. 22, no. 3, pp. 350–357, 2020, [Online]. Available: https://sid.ir/paper/734044/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top