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Information Journal Paper

Title

Reactive oxygen species mediates TNF-α-induced inflammatory response in bone marrow mesenchymal cells

Pages

  1296-1301

Abstract

 Objective(s): It is generally believed that the iNFlammatory response in bone marrow mesenchymal stem cells (BMSCs) transplantation leads to poor survival and unsatisfactory effects, and is mainly mediated by cytokines, including interleukin-1β (IL-1β ), tumor necrosis factor-α (TNF-α ). In this study, we explored the mechanisms underlying the TNF-α-induced iNFlammatory response in BMSCs. Materials and Methods: We treated BMSCs with TNF-α (1 and 10 ng/ml) for 5 days. The expression levels of key iNFlammatory mediators were evaluated by Real-time PCR. Intracellular ROS level was measured by using a 2, 7-dichlorofluorescein diacetate (DCF-DA). Results: We found that TNF-α treatment dramatically increased the expression levels of some key iNFlammatory mediators, including IL-6, IL-1β , IFN-γ and transforming growth factor β (TGF-β ). Moreover, TNF-α induced intracellular Oxidative stress by elevating intracellular reactive oxygen species (ROS) level, which is due to the increase of lipid peroxidation, the reduction of antioxidant Glutathione (GSH) levels and the inhibition of many antioxidant enzyme activities in BMSCs. Interestingly, 5 μ M curcumin, a ROS scavenger, dramatically lowered the TNF-α-induced iNFlammatory response in BMSCs. In addition, TNF-α induced the activation of extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), p38 and their down-stream transcription factors nuclear factor kappa B (NF-κ B) pathway. Conclusion: ROS mediated the TNF-α-induced iNFlammatory response via MAPK and NF-κ B pathway, and may provide a novel strategy to prevent the iNFlammatory-dependent impairments in BMSCs.

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    APA: Copy

    Wu, Liuzhong, & Pan, Yaping. (2019). Reactive oxygen species mediates TNF-α-induced inflammatory response in bone marrow mesenchymal cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(11), 1296-1301. SID. https://sid.ir/paper/734320/en

    Vancouver: Copy

    Wu Liuzhong, Pan Yaping. Reactive oxygen species mediates TNF-α-induced inflammatory response in bone marrow mesenchymal cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(11):1296-1301. Available from: https://sid.ir/paper/734320/en

    IEEE: Copy

    Liuzhong Wu, and Yaping Pan, “Reactive oxygen species mediates TNF-α-induced inflammatory response in bone marrow mesenchymal cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 11, pp. 1296–1301, 2019, [Online]. Available: https://sid.ir/paper/734320/en

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