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Information Journal Paper

Title

Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene-delivery vector

Pages

  1026-1035

Abstract

 Objective(s): Polyethylenimine (PEI) is one of the most widely used polymers in Gene delivery. The aim of this study was to modify PEI by replacing some of its primary amines with Brevinin 2R (BR-2R) peptide in order to increase the efficiency of Gene delivery. Materials and Methods: Polyethylenimine was modified by BR-2R peptide by two different approaches; A) conjugation methods including (І ) using succinimidyl 3-(2-pyridyldithio) propionate (SPDP), (П ) EDC/ NHS protocol and (П І ) EDC/NHS+6-bromohexanoic acid protocol, and B) physical interaction method. The modified polymers were characterized for their ability of plasmid condensation, number of primary amines, size and zeta potential. The transfection efficiency and cytotoxicity were evaluated on HEK293, L929, WEHI164 and Neuro2A cell lines by green fluorescent protein (GFP)-based plasmid (pGFP) reporter gene and viability assays, respectively. Apoptosis induction ability was also evaluated via PI/Annexin V assay. Results: Polyplex had size and zeta potential between 200-270 nm and +21. 5-+28. 4 mV, respectively. All Vectors were able to condense plasmid DNA in C/P=4 (carrier-plasmid ratio). Transfection results on the Neuro2A cell line showed that the Vector containing the BR-2R peptide, which was synthesized using EDC-NHS protocol had the best transfection efficiency. Conclusion: Our results showed that conjugation of Brevinin 2R as Cell penetrating peptide to Polyethyleneimine could enhance the transfection ability of the polymer.

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    Cite

    APA: Copy

    Zohrab, Fatemeh, ASOODEH, AHMAD, Jalili, Amin, Darroudi, Majid, & KAZEMI OSKUEE, REZA. (2019). Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene-delivery vector. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(9), 1026-1035. SID. https://sid.ir/paper/734715/en

    Vancouver: Copy

    Zohrab Fatemeh, ASOODEH AHMAD, Jalili Amin, Darroudi Majid, KAZEMI OSKUEE REZA. Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene-delivery vector. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(9):1026-1035. Available from: https://sid.ir/paper/734715/en

    IEEE: Copy

    Fatemeh Zohrab, AHMAD ASOODEH, Amin Jalili, Majid Darroudi, and REZA KAZEMI OSKUEE, “Brevinin-2R-linked polyethylenimine as a promising hybrid nanogene-delivery vector,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 9, pp. 1026–1035, 2019, [Online]. Available: https://sid.ir/paper/734715/en

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