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Information Journal Paper

Title

Inhibition of Akt phosphorylation attenuates resistance to TNF-α cytotoxic effects in MCF-7 cells, but not in their doxorubicin resistant derivatives

Pages

  1363-1367

Abstract

 Objective(s): Acquisition of TNF-α resistance plays role in the onset and growth of malignant tumors. Previous studies have demonstrated that MCF-7 cell line and its doxorubicin resistant variant MCF-7/Adr are resistant against the cytotoxic effects of TNF-α . In this study, we investigated the role of Akt activation in resistance of MCF-7 and MCF-7/Adr against TNF-α cytotoxicity. Materials and Methods: The role of Akt activation in TNF-α cytotoxicity was investigated by MTT cell viability assay following treatment of the cells with the chemical inhibitor of Akt activation with or without TNF-α treatment. Phosphorylation of Akt at Ser473 before and after 72 hr TNF-α treatment was also determined by western blot. Results: TNF-α treatment led to enhancement of Akt Ser473 phosphorylation. Treatment of MCF-7 cells with TNF-α along with Akt-inhibitor agent, tricribine, attenuated Akt Ser473 phosphorylation and sensitized these cells to the cytotoxic effects of TNF-α in a dose and time dependent manner while tricribine treatment did not cause any significant cytotoxicity in MCF-7/Adr cells alone or in combination with TNF-α . Conclusion: These results demonstrate that Akt phosphorylation plays pivotal role in the resistance of MCF-7 cells against TNF-α-induced cytotoxicity while it might play no significant role in the resistance of MCF-7/Adr cells against TNF-α .

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    APA: Copy

    GHANDADI, MORTEZA, Mohammadi, Atieh, BEHRAVAN, JAVAD, ABNOUS, KHALIL, Haj Ali, Negin, EHTESHAM GHARAEE, MELIKA, & MOSAFFA, FATEMEH. (2016). Inhibition of Akt phosphorylation attenuates resistance to TNF-α cytotoxic effects in MCF-7 cells, but not in their doxorubicin resistant derivatives. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(12), 1363-1367. SID. https://sid.ir/paper/735187/en

    Vancouver: Copy

    GHANDADI MORTEZA, Mohammadi Atieh, BEHRAVAN JAVAD, ABNOUS KHALIL, Haj Ali Negin, EHTESHAM GHARAEE MELIKA, MOSAFFA FATEMEH. Inhibition of Akt phosphorylation attenuates resistance to TNF-α cytotoxic effects in MCF-7 cells, but not in their doxorubicin resistant derivatives. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(12):1363-1367. Available from: https://sid.ir/paper/735187/en

    IEEE: Copy

    MORTEZA GHANDADI, Atieh Mohammadi, JAVAD BEHRAVAN, KHALIL ABNOUS, Negin Haj Ali, MELIKA EHTESHAM GHARAEE, and FATEMEH MOSAFFA, “Inhibition of Akt phosphorylation attenuates resistance to TNF-α cytotoxic effects in MCF-7 cells, but not in their doxorubicin resistant derivatives,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 12, pp. 1363–1367, 2016, [Online]. Available: https://sid.ir/paper/735187/en

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