EVALUATION OF THE EXPRESSION OF RECOMBINANT TYPE A BOTULINUM NEUROTOXIN LIGHT CHAIN LOADED ONTO A CELL-PENETRATING PEPTIDE

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SAFFARIAN P.; NAJAR PEERAYE SH.; AMANI J.; IMANI FOOLADI A.A.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2016 | Volume:18 | Issue:1 Page(s): 25-30

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Title

EVALUATION OF THE EXPRESSION OF RECOMBINANT TYPE A BOTULINUM NEUROTOXIN LIGHT CHAIN LOADED ONTO A CELL-PENETRATING PEPTIDE

Author(s)

SAFFARIAN P. | NAJAR PEERAYE SH. | AMANI J. | IMANI FOOLADI A.A. | Issue Writer Certificate

Keywords

LIGHT CHAIN OF BOTULINUM TOXIN TYPE AQ2

CELL-PENETRATING PEPTIDEQ2

RECOMBINANT PROTEINQ2

Abstract

BACKGROUND AND OBJECTIVE: Botulinum toxin type A (BoNT/A) is widely used in the treatment of some muscle contraction disorders through injection. This study aimed to produce RECOMBINANT PROTEIN through covalent bonding of the light chain of BoNT/A to the peptide TAT (47-57), and to create an optimized condition for expression and purification of the protein.METHODS: In this preliminary study, the nucleotide sequences of the light chain of BoNT/A and TAT peptide were obtained from Gen Bank, and genetic structures containing these sequences were designed and engineered. After cloning into the BL21 (DE3) E. coli vector, expression was induced by IPTG. Thereafter, optimum thermal condition and IPTG concentration for maximum expression were determined based on the difference in the intensity of staining between the bands on SDS-PAGE protein gel. The RECOMBINANT PROTEIN was purified through nickel column chromatography (Ni-NTA).FINDINGS: The produced chimeric protein is insoluble in normal conditions (1 mM IPTG and at 37oC). Through optimization of expression conditions (0.5 mM IPTG and at 18oC) 60% of the chimeric protein was produced as solution.CONCLUSION: Based on our results, through expressing the RECOMBINANT PROTEIN as a solution, the protein maintained its proper folding and function. Hence, the use of denaturation compounds for solution making and destabilization of folded protein structures is not required.

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APA: Copy

SAFFARIAN, P., NAJAR PEERAYE, SH., AMANI, J., & IMANI FOOLADI, A.A.. (2016). EVALUATION OF THE EXPRESSION OF RECOMBINANT TYPE A BOTULINUM NEUROTOXIN LIGHT CHAIN LOADED ONTO A CELL-PENETRATING PEPTIDE. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 18(1), 25-30. SID. https://sid.ir/paper/73675/en

Vancouver: Copy

SAFFARIAN P., NAJAR PEERAYE SH., AMANI J., IMANI FOOLADI A.A.. EVALUATION OF THE EXPRESSION OF RECOMBINANT TYPE A BOTULINUM NEUROTOXIN LIGHT CHAIN LOADED ONTO A CELL-PENETRATING PEPTIDE. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2016;18(1):25-30. Available from: https://sid.ir/paper/73675/en

IEEE: Copy

P. SAFFARIAN, SH. NAJAR PEERAYE, J. AMANI, and A.A. IMANI FOOLADI, “EVALUATION OF THE EXPRESSION OF RECOMBINANT TYPE A BOTULINUM NEUROTOXIN LIGHT CHAIN LOADED ONTO A CELL-PENETRATING PEPTIDE,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 18, no. 1, pp. 25–30, 2016, [Online]. Available: https://sid.ir/paper/73675/en

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